1st-2017

Permanent URI for this collectionhttp://repository.kln.ac.lk/handle/123456789/17673

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Subject: search.filters.subject.antimicrobial

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    A comparative study on the anti-microbial efficacy of two Ayurveda powders (Prathisarana) used in periodontal diseases
    (Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Wijebandara, D.M.K.K.; Peiris, K.P.P.; Karunarathna, E.D.C.; Sudesh, A.D.H.
    Kushtadi (KU) and Karanjadi (KR) are prominently used Ayurvedic powders (Prathisarana) in the management of periodontal diseases. This study was carried out for the determination of antimicrobial efcacy of these two drugs using standard Antimicrobial Sensitivity Test against Candida albicans and a fungal culture isolated from a patient. The Antimicrobial Sensitivity Test was performed according to the Well diffusion method having 6mm diameter wells on Sabouraud Dextrose Agar (SDA). Each well was loaded with test drugs mixed in 20μl of sterile distilled water and uconazole 2.5mg / ml as the positive control. According to the results, KR showed an average Inhibition Zone Diameter (IZD) of 20mm for the isolated culture while KU did not show any inhibition. The results were similar for Candida albicans, KR showing IZD 18 mm, KU IZD 0 mm while positive control showed an inhibition of 25 mm. Therefore, it can be concluded that both Candida albicans and the isolated culture were sensitive only for KR making it the only effective drug out of the two drugs tested in this study.
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    Microbiological study of an indigenous mouth wash (Gandusha) used in periodontal diseases.
    (Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Dewasurendra, D.M.W.; Peiris, K.P.P.; Karunarathna, E.D.C.; Sudesh, A.D.H.
    Mouth wash (Gandusha) is clinically effective in the management of periodontal diseases. This study is an in-vitro analysis on determining the antimicrobial efcacy of this drug using standard Antimicrobial Sensitivity Test against a fungal culture isolated from a patient. The test was performed according to the Well diffusion method having 6mm diameter wells on Sabouraud Dextrose Agar (SDA). Each well was loaded with the test drug mixed in 20μl of sterile distilled water and uconazole 2.5mg / ml was used as the positive control. According to the results, the test drug showed an average Inhibition Zone Diameter (IZD) of 19mm while the positive control showed IZD of 15mm. Therefore, it can be stated that the test drug is more effective than the positive control and the isolated culture is sensitive to the test drug as it had shown an IZD of 19mm while it is intermediate sensitive for the positive control as 15mm comes under Intermediate sensitive range (15-18mm). These results support the claim that the indigenous mouth wash (Gandusha) is effective in the management of periodontal diseases.
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    An analytical study on the antimicrobial activity of Visarpahara Tailaya against Candida albicans and Staphylococcus aureus.
    (Department of Shalya Shalakya,Gampaha Wickramarachchi Ayurveda Institute,University of Kelaniya,Sri Lanka, 2017) Madumali, M.G.B.; Wickramarachchi, W.J.; Karunarathna, E.D.C.; Sudesh, A.D.H.
    Visarpahara Tailaya is a herbo-mineral preparation recommended to use clinically in the management of eczema, scabies, itches and other types of skin diseases mainly caused by bacteria and fungi. This study was conducted to determine the antimicrobial activity of this drug using the standard Antimicrobial Sensitivity Test against Staphylococcus aureus and Candida albicans. The test was performed according to the Well diffusion Method having 6 mm diameter wells on Mueller Hinton Agar (MHA) for S. aureus and Sabouraud Dextrose Agar (SDA) for C. albicans. As the positive controls, Amoxicillin 10mg/ml was used for S.aureus and Fluconazole 2.5mg /ml for C. albicans. According to the results, the test drug showed an average Inhibition Zone Diameter (IZD) of 12mm for S. aureus and 17mm for C. albicans while the positive controls showed 51mm and 25mm respectively .These results show that the test drug Visarpahara Thailaya is effective for both test organisms with an increased effectiveness for C.albicans compared to S. aureus. Therefore, it is concluded that Visarpahara Tailaya can be effectively used in the management of C. albicans while it is less effective for S. aureus.