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Tetravalent dengue specific domain III based chimeric recombinant protein as dengue diagnostic intermediates for the detection of both anti-dengue immunoglobulin M(IGM) and imunoglobulin G(IGG) antibodies in human serum samples.

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dc.contributor.author Hapugoda, M.D. en_US
dc.contributor.author Abeyewickreme, W. en_US
dc.contributor.author Gunasena, S. en_US
dc.contributor.author Khanna, N. en_US
dc.date.accessioned 2015-08-29T15:40:03Z en_US
dc.date.available 2015-08-29T15:40:03Z en_US
dc.date.issued 2006 en_US
dc.identifier.citation Proceedings of the Symposium,and Workshop on Water Environment and Health Research in Sri Lanka. 2006; 1:13 en_US
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/9358 en_US
dc.description Proceedings of the Symposium,and Workshop on Water Environment and Health Research in Sri Lanka.to Commemorate the work of Dr Felix Prashantha Amerasinghe(1948-2005), , 28-29 August, 2006, International Water Management Institute, Colombo, Sri Lanka en_US
dc.description.abstract BACKGROUND: Dengue infection is an important mosquito borne viral infection caused by four serotypes of dengue virus with explosive outbreaks occurring in many tropical areas. Laboratory diagnosis of the disease mainly depends on Enzyme-Linked Immunosorbent Assay (ELISA) based on whole viral antigens which cause biohazard risk, high production cost and cross reactivity with other flaviviruses. OBJECTIVES: To produce a recombinant protein antigen to overcome problems associated with whole dengue viral antigen/lysate or recombinant whole envelope protein. STUDY DESIGN: We have designed and expressed a single recombinant tetravalent protein antigen which contains Domain III of envelope protein from all four serotypes of dengue virus, linked with each other through penta glycine linkers. This synthetic gene was expressed in Escherichia coli and protein was purified using a single affinity chromatographic step. We developed Immunoglobulin M (IgM) and Immunoglobulin G (IgG) ELISAs using this novel protein as the capture antigen. The antigen was validated as a diagnostic reagent on serum samples. RESULTS: 30 mg of recombinant protein per litre of culture could be purified. Both ELISAs developed using this novel recombinant protein showed an excellent agreement with a commercially available IgM ELISA (MRL diagnostic) and haernagglutination inhibition assay respectively. Conclusions: Findings of this study suggests that this single dengue specific tetravalent recombinant protein antigen can be used as a diagnostic intermediate for detection of dengue infection. en_US
dc.language.iso en_US en_US
dc.publisher International Water Management Institute en_US
dc.subject Dengue en_US
dc.subject Dengue-diagnosis en_US
dc.subject.mesh Recombinant Fusion Proteins en_US
dc.subject.mesh Enzyme-Linked Immunosorbent Assay en_US
dc.title Tetravalent dengue specific domain III based chimeric recombinant protein as dengue diagnostic intermediates for the detection of both anti-dengue immunoglobulin M(IGM) and imunoglobulin G(IGG) antibodies in human serum samples. en_US
dc.type Conference Abstract en_US
dc.identifier.department Molecular Medicine Unit en_US
dc.identifier.department Parasitology en_US
dc.creator.corporateauthor International Water Management Institute en_US
dc.creator.corporateauthor National Science Foundation en_US
dc.creator.corporateauthor World Health Organization en_US


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