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A Comparative field study of novel commercial Antigen Detection Enzyme-Linked Immunosorbent Assay (ELISA) with Reverse Transcription Polymerase Chain Reaction (RT- PCR) assay for early definitive laboratory diagnosis of dengue viral infection in Sri Lanka

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dc.contributor.author Hapugoda, M.D.
dc.contributor.author Jayasooriya, D.H.S.W.
dc.contributor.author Gunawardene, Y.I.N.S.
dc.contributor.author Wellawaththage, C.
dc.contributor.author Premaratna, R.
dc.contributor.author Abeyewickreme, W.
dc.date.accessioned 2015-08-01T12:14:35Z
dc.date.available 2015-08-01T12:14:35Z
dc.date.issued 2007
dc.identifier.citation Proceedings of the Sri Lanka Association for the Advancement of Science. 2007; 63 (Part I): 6 en_US
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/9082
dc.description Sri Lanka Association for the Advancement of Science, 63rd Annual Sessions Abstracts(008A), December 2007. Colombo en_US
dc.description.abstract Dengue is an important mosquito borne viral infection in South East Asia. Early definitive laboratory diagnosis of infection would help in management of patients and reducing the case fatality rate. The objective of this study was to determine the accuracy of novel commercial Antigen Detection Enzyme-Linked Immunosorbent Assay (ELISA) using Non Structural protein 1 (NS1) (Bio Rad) for early definitive laboratory diagnosis of dengue infection under field conditions in Sri Lanka. A panel of acute serum samples collected from 99 patients clinically suspected of having dengue fever (<5 days) warded at the North Colombo Teaching Hospital, Ragama, Sri Lanka were used for the present study. Serum samples were tested using Antigen Detection ELISA according to the method described by the manufacturer. Results of this novel assay were compared with RT-PCR assay using Chi-squared test. Two variables were analyzed at a 95% confidence interval and P value <0.05 was considered as significant. Twenty two and 65 patients were positive and negative, respectively, for dengue infection by both assays. Nine patients were confirmed as dengue by the Antigen Detection ELISA only. Three patients were confirmed as dengue by RT-PCR assay only. Antigen detection ELISA showed 88% of agreement with the RT-PCR assay. According to the Chi-squared test, there was no significant difference between the two assays for early diagnosis of dengue infection (?2=46, P=0.0000). Novel commercial Antigen Detection ELISA kit (Bio-Rad 72830) can be used for early definitive laboratory diagnosis of dengue infection in Sri Lanka under field conditions. Acknowledgement: the International Atomic Energy Agency (SRL 06/28) for technical co-operation and APCOT Marketing LTD, Sri Lanka for supplying Antigen detection ELISA kits. en_US
dc.language.iso en en_US
dc.publisher Sri Lanka Association for the Advancement of Science en_US
dc.subject Dengue en_US
dc.subject Polymerase Chain Reaction-methods en_US
dc.subject Reverse Transcriptase Polymerase Chain Reaction-methods en_US
dc.subject Enzyme-Linked Immunosorbent Assay-methods en_US
dc.title A Comparative field study of novel commercial Antigen Detection Enzyme-Linked Immunosorbent Assay (ELISA) with Reverse Transcription Polymerase Chain Reaction (RT- PCR) assay for early definitive laboratory diagnosis of dengue viral infection in Sri Lanka en_US
dc.type Conference Abstract en_US


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