dc.contributor.author |
Hapuarachchi, H.A.C. |
en_US |
dc.contributor.author |
Dayanath, M.Y.D. |
en_US |
dc.contributor.author |
Bandara, K.B.A.T. |
en_US |
dc.contributor.author |
Abeysundara, S. |
en_US |
dc.contributor.author |
Abeyewickreme, W. |
en_US |
dc.contributor.author |
de Silva, N.R. |
en_US |
dc.contributor.author |
Hunt, S.Y. |
en_US |
dc.contributor.author |
Sibley, C.H. |
en_US |
dc.date.accessioned |
2014-10-29T09:24:21Z |
|
dc.date.available |
2014-10-29T09:24:21Z |
|
dc.date.issued |
2006 |
en_US |
dc.identifier.citation |
The American Journal of Tropical Medicine and Hygiene. 2006; 74(2): pp.198-204 |
en_US |
dc.identifier.issn |
0002-9637 (Print) |
en_US |
dc.identifier.issn |
1476-1645 (Electronic) |
en_US |
dc.identifier.uri |
http://repository.kln.ac.lk/handle/123456789/1726 |
|
dc.description |
Indexed in MEDLINE |
|
dc.description.abstract |
Sulfadoxine-pyrimethamine (SP) is the second-line treatment for Plasmodium falciparum malaria in Sri Lanka. Resistance to SP is caused by point mutations in the dihydrofolate reductase (Pf-dhfr) and dihydropteroate synthase (Pf-dhps) genes of P. falciparum. We determined the genotype of Pf-dhfr and Pf-dhps and the clinical response to SP in 30 field isolates of P. falciparum from Sri Lanka. All patients treated with SP had an adequate clinical response. Eighty-five percent (23 of 27) of pure field isolates carried parasites with double mutant alleles of Pf-dhfr (C59R + S108N) and showed about 200-fold higher levels of resistance to pyrimethamine than the wild type in a yeast system. None of the isolates had either known or novel mutations at other positions in the dhfr domain. In contrast, 67% (20 of 30) of the isolates carried parasites that were wild type for Pf-dhps. In Sri Lanka, detection of the triple mutant allele of Pf-dhfr will require tracking mutations at codon 51 |
|
dc.publisher |
American Society of Tropical Medicine and Hygiene |
en_US |
dc.title |
Point mutations in the dihydrofolate reductase and dihydropteroate synthase genes of Plasmodium falciparum and resistance to sulfadoxine-pyrimethamine in Sri Lanka |
en_US |
dc.type |
Article |
en_US |
dc.identifier.department |
Parasitology |
en_US |
dc.creator.corporateauthor |
American Society of Tropical Medicine and Hygiene |
en_US |