dc.contributor.author |
Faleel, F.D. |
en_US |
dc.contributor.author |
Zoysa, M.I. |
en_US |
dc.contributor.author |
Lokuhetti, M.D. |
en_US |
dc.contributor.author |
Gunawardene, Y.I.N.S. |
en_US |
dc.contributor.author |
Chandrasekharan, N.V. |
en_US |
dc.contributor.author |
Dassanayake, R.S. |
en_US |
dc.date.accessioned |
2017-02-13T05:20:49Z |
en_US |
dc.date.available |
2017-02-13T05:20:49Z |
en_US |
dc.date.issued |
2016 |
en_US |
dc.identifier.citation |
Journal of Cancer Research and Therapeutics. 2016;12(4):1272-1277 |
en_US |
dc.identifier.issn |
0973-1482 (Print) |
en_US |
dc.identifier.issn |
1998-4138 (Electronic) |
en_US |
dc.identifier.issn |
1998-4138 (Linking) |
en_US |
dc.identifier.uri |
http://repository.kln.ac.lk/handle/123456789/16389 |
en_US |
dc.description |
Indexed In MEDLINE |
en_US |
dc.description.abstract |
AIM: Mutations in K-ras codon 12 and 13 of exon 2 are known to affect prognosis and impart resistance to anti-epidermal growth factor monoclonal antibody therapy in colorectal carcinoma (CRC). Our aim was to investigate the utility value of modified mismatch polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay to detect mutation in K-ras codons of CRC patients and to relate the mutational status to liver metastasis. METHODOLOGY: Mismatch PCR-RFLP was developed to detect K-ras mutations in DNA isolated from paraffinized tumor tissue of thirty CRC patients. All patients had 5 year follow-up data to detect liver metastasis. Cross-tabulations were generated between K-ras mutations and the metastatic status. The Chi-square test was used to indicate statistical significance of the association. RESULTS: Of the 30 CRC patients investigated, K-ras mutations of codons 12 and/or 13 of exon 2 were detected in 14 (46.6%). Meanwhile, 13 patients (43.3%) were observed to have developed liver metastases. There was a significant association between the presence of the K-ras mutation in codon 12 and the occurrence of liver metastasis (χ2 = 4.693, P = 0.030) on the contrary to the mutation in codon 13 to which such occurrence of liver metastases was not seen (χ2 = 1.884, P = 0.169). CONCLUSION: Codon 12 of exon 2 of K--ras gene detected by modified mismatch PCR-RFLP assay is significantly associated with liver metastasis in CRC patients during the first 5 years after surgery. Thus, modified mismatch PCR-RFLP protocol is a suitable method in this setting to detect K-ras gene mutations predicting liver metastasis in CRC patients. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.publisher |
Medknow Publications |
en_US |
dc.subject |
Colorectal Neoplasms |
en_US |
dc.subject.mesh |
Colorectal Neoplasms-genetics |
en_US |
dc.subject.mesh |
Colorectal Neoplasms-pathology |
en_US |
dc.subject.mesh |
Colorectal Neoplasms-surgery |
en_US |
dc.subject.mesh |
Codon |
en_US |
dc.subject.mesh |
Exons |
en_US |
dc.subject.mesh |
Genes, ras |
en_US |
dc.subject.mesh |
Genetic Predisposition to Disease |
en_US |
dc.subject.mesh |
Mutation |
en_US |
dc.subject.mesh |
Liver Neoplasms-secondary |
en_US |
dc.subject.mesh |
Polymerase Chain Reaction |
en_US |
dc.subject.mesh |
Follow-Up Studies |
en_US |
dc.title |
Modified mismatch polymerase chain reaction-restriction fragment length polymorphism detected mutations in codon 12 and 13 of exon 2 of K-ras gene in colorectal cancer patients and its association with liver metastases: Data from a South Asian country |
en_US |
dc.type |
Article |
en_US |
dc.creator.corporateauthor |
Association of Radiation Oncologists of India (AROI) |
en_US |