dc.contributor.author |
Chandrasena, T.G.A.N. |
en_US |
dc.contributor.author |
Premaratna, R. |
en_US |
dc.contributor.author |
Abeyewickreme, W. |
en_US |
dc.contributor.author |
de Silva, N.R. |
en_US |
dc.date.accessioned |
2014-10-29T09:19:22Z |
|
dc.date.available |
2014-10-29T09:19:22Z |
|
dc.date.issued |
2002 |
en_US |
dc.identifier.citation |
Transactions of the Royal Society of Tropical Medicine and Hygiene. 2002; 96(1): pp.60-63 |
en_US |
dc.identifier.issn |
0035-9203 (Print) |
en_US |
dc.identifier.issn |
1878-3503 (Electronic) |
en_US |
dc.identifier.uri |
http://repository.kln.ac.lk/handle/123456789/1496 |
|
dc.description |
Indexed in MEDLINE |
|
dc.description.abstract |
The sensitivity, specificity and cost effectiveness of an immunochromatographic card test (ICT, AMRAD) for the diagnosis of bancroftian filariasis were estimated against 2 standard parasitological techniques: thick blood film (TBF) and Nuclepore membrane filtration (NMF). Individuals were selected from endemic localities in the Western Province (n = 213) and from the non-endemic Central Province (n = 29) of Sri Lanka. Blood was collected between 21:00 and midnight. Sixty microlitre of non-heparinized blood, and 1 mL and 100 microL of heparinized blood were used in TBF, NMF andICT, respectively. NMF was positive in 31.5% (67/213) of the endemic group, with a mean microfilaria (mf) count of 343/mL (range 8-1782, SD 422). All 67 were positive by ICT (sensitivity 100%), but only 63 by TBF (sensitivity 94%). Among the endemic population there were 12 who were mf negative but antigen positive by ICT. There were, however, no false positives among the non-endemic controls, indicating the possibility that the ICTmay in fact be more sensitive and 100% specific. Thus, ICT filariasis test appears to be more effective (both sensitive and specific) than TBF or NMF in diagnosing infection in lymphatic filariasis. The direct unit recurrent costs of the 2 survey tools, TBF and ICT, were US$ 0.30 (Rs. 27/=) and US$ 2.75 (Rs. 248/=), respectively. The high cost of the ICT may be offset by other factors that are difficult to cost. |
en_US |
dc.publisher |
Oxford University Press |
en_US |
dc.subject |
Filariasis |
en_US |
dc.subject |
Clinical Trial |
en_US |
dc.subject |
Comparative Study |
en_US |
dc.subject |
Controlled Clinical Trial |
en_US |
dc.subject |
Antigens, Helminth-blood |
en_US |
dc.subject |
Cost-Benefit Analysis |
en_US |
dc.subject |
Filariasis-diagnosis |
en_US |
dc.subject |
Reagent Kits, Diagnostic-standards |
en_US |
dc.subject |
Wuchereria bancrofti-isolation and purification |
en_US |
dc.title |
Evaluation of the ICT whole-blood antigen card test to detect infection due to Wuchereria bancrofti in Sri Lanka |
en_US |
dc.type |
Article |
en_US |
dc.identifier.department |
Parasitology |
en_US |
dc.identifier.department |
Medicine |
en_US |
dc.creator.corporateauthor |
Royal Society of Tropical Medicine and Hygiene |
en_US |