dc.contributor.author |
de Silva, D.G.H. |
en_US |
dc.contributor.author |
Candy, D.C. |
en_US |
dc.contributor.author |
Mendis, L.N. |
en_US |
dc.contributor.author |
Chart, H. |
en_US |
dc.contributor.author |
Rowe, B. |
en_US |
dc.date.accessioned |
2014-10-29T09:10:33Z |
|
dc.date.available |
2014-10-29T09:10:33Z |
|
dc.date.issued |
1992 |
en_US |
dc.identifier.citation |
The Journal of Infection. 1992; 25(3): pp.273-278 |
en_US |
dc.identifier.issn |
0163-4453 (Print) |
en_US |
dc.identifier.issn |
1532-2742 (Electronic) |
en_US |
dc.identifier.uri |
http://repository.kln.ac.lk/handle/123456789/1167 |
|
dc.description |
Indexed in MEDLINE |
|
dc.description.abstract |
A total of 192 samples of serum from 113 Sri Lankan patients with clinical dysentery was examined for antibodies of the IgM class to the lipopolysaccharides (LPSs) of Shigella dysenteriae-1 and Escherichia coli O157:H7. By means of ELISA and immunoblotting, 59 patients were found to have serum antibodies to the LPS of S. dysenteriae-1 only. Four samples from one patient were found to contain serum antibodies to the LPSs of both S. dysenteriae-1 and E. coli O157:H7. Antibodies to the LPS of S. dysenteriae-1 were also detected in 16 samples from 25 children, from Sri Lanka, with no previous history of dysentery; one of these children also had antibodies to the LPS of E. coli O157:H7. Analysis of 16 samples from apparently healthy children in the U.K. showed that only one serum contained antibodies to the LPS of S. dysenteriae-1. This patient had a history of recent travel to Pakistan. The isolation of S. dysenteriae-1 remains the preferred test for the diagnosis of bacillary dysentery. The use of serology as a means of providing evidence of infection with S. dysenteriae-1, however may prove to be a useful adjunct to cultural techniques but needs to be validated in an area where this organism is endemic |
en_US |
dc.publisher |
Elsevier-W.B. Saunders |
en_US |
dc.subject |
Shigella dysenteriae |
|
dc.subject |
Shigella dysenteriae-classification |
|
dc.subject |
Dysentery, Bacillary-diagnosis |
|
dc.subject |
Electrophoresis, Polyacrylamide Gel |
|
dc.subject |
Enzyme-Linked Immunosorbent Assay |
|
dc.subject |
Escherichia coli-isolation and purification |
|
dc.subject |
Immunoglobulin M-immunology |
|
dc.subject |
Lipopolysaccharides-immunology |
|
dc.title |
Serological diagnosis of infection by Shigella dysenteriae-1 in patients with bacillary dysentery. |
en_US |
dc.type |
Article |
en_US |
dc.identifier.department |
Paediatrics |
en_US |
dc.creator.corporateauthor |
British Society for the Study of Infection |
en_US |