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Title: | Effects of high external concentrations of K+ on 86Rb+ efflux in human platelets: evidence for Na+/K+/2Cl- co-transport. |
Authors: | de Silva, H.A. Carver, J.G. Aronson, J.K. |
Keywords: | Biological Transport-drug effects Blood Platelets-metabolism Bumetanide-pharmacology Carboxylic Acids-pharmacology Chlorine-blood Indenes-pharmacology Ouabain-pharmacology Potassium-blood Rubidium Radioisotopes-blood Sodium-blood |
Issue Date: | 1996 |
Publisher: | Portland Press on behalf of the Medical Research Society and the Biochemical Society |
Citation: | Clinical Science. 1996; 91(6): pp.725-731 |
Abstract: | Na+/K+/2Cl- co-transport mediates a bidirectional symport of Na+, K+ and Cl-. The important properties of the co-transport system are its requirement for Na+, K+ and Cl- and its inhibition by loop diuretics such as bumetanide. This co-transporter has been described in a number of animal and human tissues. However, its presence in human platelets, although inferred, has not been demonstrated directly. 2. We have studied the efflux of 86Rb+ (a marker for K+) from Rb(+)-loaded platelets, and have defined their response to stimulation by high concentrations of external K+. 3. KCl (30-120 mmol/l) stimulated a concentration-dependent increase in 86Rb+ efflux from the platelets. This efflux was completely inhibited by bumetanide (10 mumol/l) but was insensitive to ouabain and R(+)-[(dihydroindenyl)oxy]alkanoic acid. It also required Cl- in the external medium, but did not depend on the presence of extracellular Na+. 4. These observations suggest that 86Rb+ efflux from platelets stimulated by external K+ occurs via Na+/K+/2Cl- co-transport acting in a K+/K+ (K+/Rb+) exchange mode. 5. Non-stimulated efflux of 86Rb+ from the platelets (i.e. in the presence of 5 mmol/l K+) had the characteristics of Na+/K+/2Cl- co-transport acting in normal mode. |
Description: | Indexed in MEDLINE |
URI: | http://repository.kln.ac.lk/handle/123456789/1278 |
ISSN: | 0143-5221 (Print) 1470-8736 (Electronic) |
Appears in Collections: | Journal/Magazine Articles |
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