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DC Field | Value | Language |
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dc.contributor.author | de Silva, H.A. | en_US |
dc.contributor.author | Carver, J.G. | en_US |
dc.contributor.author | Aronson, J.K. | en_US |
dc.date.accessioned | 2014-10-29T09:14:44Z | - |
dc.date.available | 2014-10-29T09:14:44Z | - |
dc.date.issued | 1996 | en_US |
dc.identifier.citation | Clinical Science. 1996; 91(6): pp.725-731 | en_US |
dc.identifier.issn | 0143-5221 (Print) | en_US |
dc.identifier.issn | 1470-8736 (Electronic) | en_US |
dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/1278 | - |
dc.description | Indexed in MEDLINE | - |
dc.description.abstract | Na+/K+/2Cl- co-transport mediates a bidirectional symport of Na+, K+ and Cl-. The important properties of the co-transport system are its requirement for Na+, K+ and Cl- and its inhibition by loop diuretics such as bumetanide. This co-transporter has been described in a number of animal and human tissues. However, its presence in human platelets, although inferred, has not been demonstrated directly. 2. We have studied the efflux of 86Rb+ (a marker for K+) from Rb(+)-loaded platelets, and have defined their response to stimulation by high concentrations of external K+. 3. KCl (30-120 mmol/l) stimulated a concentration-dependent increase in 86Rb+ efflux from the platelets. This efflux was completely inhibited by bumetanide (10 mumol/l) but was insensitive to ouabain and R(+)-[(dihydroindenyl)oxy]alkanoic acid. It also required Cl- in the external medium, but did not depend on the presence of extracellular Na+. 4. These observations suggest that 86Rb+ efflux from platelets stimulated by external K+ occurs via Na+/K+/2Cl- co-transport acting in a K+/K+ (K+/Rb+) exchange mode. 5. Non-stimulated efflux of 86Rb+ from the platelets (i.e. in the presence of 5 mmol/l K+) had the characteristics of Na+/K+/2Cl- co-transport acting in normal mode. | - |
dc.publisher | Portland Press on behalf of the Medical Research Society and the Biochemical Society | en_US |
dc.subject | Biological Transport-drug effects | - |
dc.subject | Blood Platelets-metabolism | - |
dc.subject | Bumetanide-pharmacology | - |
dc.subject | Carboxylic Acids-pharmacology | - |
dc.subject | Chlorine-blood | - |
dc.subject | Indenes-pharmacology | - |
dc.subject | Ouabain-pharmacology | - |
dc.subject | Potassium-blood | - |
dc.subject | Rubidium Radioisotopes-blood | - |
dc.subject | Sodium-blood | - |
dc.title | Effects of high external concentrations of K+ on 86Rb+ efflux in human platelets: evidence for Na+/K+/2Cl- co-transport. | en_US |
dc.type | Article | en_US |
dc.identifier.department | Pharmacology | en_US |
dc.creator.corporateauthor | Biochemical Society (Great Britain) | en_US |
dc.creator.corporateauthor | Medical Research Society (Great Britain) | en_US |
dc.creator.corporateauthor | International Society of Hypertension | en_US |
Appears in Collections: | Journal/Magazine Articles |
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