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Title: | Dirofilaria repens: Cloning and Characterization of a Repeated DNA Sequence for the Diagnosis of Dirofilariasis in Dogs, Canis familiaris |
Authors: | Chandrasekharan, N.V. Karunanayake, E.H. Franzen, L. Abeyewickreme, W. Pettersson, U. |
Keywords: | Dirofilariasis Dirofilaria-genetics Dirofilaria-isolation and purification Dirofilariasis-diagnosis Dog Diseases-diagnosis DNA-analysis DNA-chemistry DNA Probes-chemistry Repetitive Sequences, Nucleic Acid |
Issue Date: | 1994 |
Publisher: | Academic Press |
Citation: | Experimental Parasitology. 1994; 78(3): pp.279-286 |
Abstract: | A highly repetitive DNA element from the genome of the filarial nematode Dirofilaria repens has been cloned and sequenced. The 176-base pair repeating units are arranged in direct tandem and are clustered in the parasite genome. All repeats appear to belong to a single family although some elements have diverged significantly. The repeats are present in about 15,000 copies and constitute approximately 3.0% of the parasite genome. The cloned repetitive sequence hybridized only to D. repens DNA and was sensitive enough to detect 250 to 500 pg of D. repens DNA, a single microfilariae in infected blood samples, and a single third stage larvae in mosquitoes. The high specificity and sensitivity of the cloned fragment makes it ideal as a diagnostic probe for detecting D. repens in both the host and the vector. |
Description: | Indexed in MEDLINE |
URI: | http://repository.kln.ac.lk/handle/123456789/1215 |
ISSN: | 0014-4894 (Print) 1090-2449 (Electronic) |
Appears in Collections: | Journal/Magazine Articles |
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