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DC Field | Value | Language |
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dc.contributor.author | Chandrasekharan, N.V. | en_US |
dc.contributor.author | Karunanayake, E.H. | en_US |
dc.contributor.author | Franzen, L. | en_US |
dc.contributor.author | Abeyewickreme, W. | en_US |
dc.contributor.author | Pettersson, U. | en_US |
dc.date.accessioned | 2014-10-29T09:11:25Z | |
dc.date.available | 2014-10-29T09:11:25Z | |
dc.date.issued | 1994 | en_US |
dc.identifier.citation | Experimental Parasitology. 1994; 78(3): pp.279-286 | en_US |
dc.identifier.issn | 0014-4894 (Print) | en_US |
dc.identifier.issn | 1090-2449 (Electronic) | en_US |
dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/1215 | |
dc.description | Indexed in MEDLINE | |
dc.description.abstract | A highly repetitive DNA element from the genome of the filarial nematode Dirofilaria repens has been cloned and sequenced. The 176-base pair repeating units are arranged in direct tandem and are clustered in the parasite genome. All repeats appear to belong to a single family although some elements have diverged significantly. The repeats are present in about 15,000 copies and constitute approximately 3.0% of the parasite genome. The cloned repetitive sequence hybridized only to D. repens DNA and was sensitive enough to detect 250 to 500 pg of D. repens DNA, a single microfilariae in infected blood samples, and a single third stage larvae in mosquitoes. The high specificity and sensitivity of the cloned fragment makes it ideal as a diagnostic probe for detecting D. repens in both the host and the vector. | en_US |
dc.publisher | Academic Press | en_US |
dc.subject | Dirofilariasis | |
dc.subject | Dirofilaria-genetics | |
dc.subject | Dirofilaria-isolation and purification | |
dc.subject | Dirofilariasis-diagnosis | |
dc.subject | Dog Diseases-diagnosis | |
dc.subject | DNA-analysis | |
dc.subject | DNA-chemistry | |
dc.subject | DNA Probes-chemistry | |
dc.subject | Repetitive Sequences, Nucleic Acid | |
dc.title | Dirofilaria repens: Cloning and Characterization of a Repeated DNA Sequence for the Diagnosis of Dirofilariasis in Dogs, Canis familiaris | en_US |
dc.type | Article | en_US |
dc.identifier.department | Parasitology | en_US |
Appears in Collections: | Journal/Magazine Articles |
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