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Paediatric rota-virus diarrhoea in Sri Lanka: a preliminary report

Show simple item record Chandrasena, T.G.A.N. Rajindrajith, S. Ahmed, K. Pathmeswaran, A. Abeyewickreme, W. Nakagomi, O. 2015-09-16T04:36:13Z 2015-09-16T04:36:13Z 2007
dc.identifier.citation The Ceylon Medical Journal. 2007; 52(Supplement 1):17 en_US
dc.identifier.issn 0009-0875 (Print)
dc.description Oral Presentation Abstract (OP23), 120th Annual Scientific Sessions, Sri Lanka Medical Association, 2007 Colombo, Sri Lanka en_US
dc.description.abstract OBJECTIVE: To determine the prevalence, severity and molecular epidemiology of group A rotavirus infections among children hospitalized with diarrhoea in Sri Lanka. DESIGN, SETTINGS AND METHODS: A prospective hospital-based study was conducted in the paediatric units of the Colombo North Teaching Hospital from April 2005 to February 2006. Stool samples of children admitted with diarrhoea were analysed for Group A rotavirus antigen by enzyme linked immunosorbent assay (ELISA) (Rotaclone ®). Samples positive for rotavirus were characterised by electropherotyping (PAGE) and serotyping (reverse transcription-polymerase chain reaction (RT-PCR) respectively. Severity of diarrhoea was assessed by the Vesikari severity score. RESULTS: A total of 341 children [(204 males, mean age 25.7 months (range 1-144)] were studied. Sixty seven (19.6%) had rotavirus diarrhoea. RT-PCR and PAGE were done on 58 rotavirus positive samples. Thirty one samples were PAGE positive with 6 different electropherotypes. RT-PCR revealed the presence of serotypes Gl, G2, G3, G4 and G9 in 7 (12.1%), 16 (27.6%), 2 (3.4%), 2 (3.4%) and 11 (19.0%) samples respectively. Twenty samples (34.5%) were untypable. Severity score assessed in 326 patients revealed a mean score of 13.3 and 11.4 in rotavirus positive and negative diarrhoeas respectively (p<0.05). Presence, frequency and duration of vomiting and duration of diarrhoea were significantly higher in rotavirus infections (p<0.05). CONCLUSIONS: Rotavirus is an important agent of severe paediatric diarrhoea in Sri Lanka. Molecular analysis indicates genetic diversity among group A rotavirus. This study reports for the first time G9 type rotavirus infection in Sri Lanka. en_US
dc.language.iso en_US en_US
dc.publisher Sri Lanka Medical Association en_US
dc.subject Paediatric rota-virus diarrhoea en_US
dc.title Paediatric rota-virus diarrhoea in Sri Lanka: a preliminary report en_US
dc.type Article en_US

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