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The use of recombinant K39, KMP11, and crude antigen-based indirect ELISA as a serological diagnostic tool and a measure of exposure for cutaneous leishmaniasis in Sri Lanka

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dc.contributor.author Karunathilake, C.
dc.contributor.author Alles, N.
dc.contributor.author Dewasurendra, R.
dc.contributor.author Weerasinghe, I.
dc.contributor.author Chandrasiri, N.
dc.contributor.author Piyasiri, S.B.
dc.contributor.author Samaranayake, N.
dc.contributor.author Silva, H.
dc.contributor.author Manamperi, N.
dc.contributor.author Karunaweera, N.
dc.date.accessioned 2024-01-08T08:22:08Z
dc.date.available 2024-01-08T08:22:08Z
dc.date.issued 2024
dc.identifier.citation Parasitology Research.2024;123(1):77. [Epub 2023 Dec 29] en_US
dc.identifier.issn 0932-0113 (Print)
dc.identifier.issn 1432-1955 (Electronic)
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/27163
dc.description Indexed in MEDLINE, Scopus, SCIE en_US
dc.description.abstract Cutaneous leishmaniasis (CL) in Sri Lanka is caused by Leishmania donovani, a parasite widely known to cause visceral leishmaniasis. Despite the fact that CL is not generally believed to elicit serological immune responses, recent studies show the presence of antibody responses against this atypical form of CL. This study assesses the potential of using recombinant K39 (rK39), KMP11, and crude parasite antigen-based indirect ELISAs as serological diagnostic tools and measures of exposure for CL in Sri Lanka. The study used serum samples from confirmed CL patients (n = 266) and apparently healthy individuals from endemic settings (n = 411). Serum samples from individuals residing in non-endemic areas were used as negative controls. In-house indirect ELISAs were optimized and validated for recombinant antigens. Previously validated crude parasite extract-based indirect ELISA was performed for comparison. The statistical analyses were performed using SPSS v26.0. The rK39 (sensitivity = 71.2%, specificity = 64%) and KMP11 (sensitivity = 79.2%, specificity = 71.4%) based indirect ELISA were shown to be less suitable for the diagnosis of CL, while crude parasite extract-based indirect ELISA (sensitivity = 82.4%, specificity = 85.7%) might be a better method of diagnosis. All 03 ELISAs seemed to be good methods as measures of exposure since correlations were observed between the seropositivity of all 03 ELISAs (rK39: p = 0.037, KMP11: p = 0.007, CrudeAg: p = 0.000) with provincial case incidences. The findings will be important in identifying the disease hotspots in order to design the control measures for CL induced by L. donovani in Sri Lanka. en_US
dc.language.iso en en_US
dc.publisher Springer International en_US
dc.subject Leishmaniasis, Cutaneous en_US
dc.subject Leishmaniasis, Cutaneous-diagnosis en_US
dc.subject Leishmaniasis, Visceral en_US
dc.subject Leishmaniasis, Visceral-diagnosis en_US
dc.subject Leishmania donovani
dc.subject Enzyme-Linked Immunosorbent Assay
dc.subject Antigens, Protozoan
dc.subject Sero-prevalence
dc.subject Sri Lanka-epidemiology
dc.title The use of recombinant K39, KMP11, and crude antigen-based indirect ELISA as a serological diagnostic tool and a measure of exposure for cutaneous leishmaniasis in Sri Lanka en_US
dc.type Article en_US


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