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Epidemiological evidence of acute transmission of zika virus infection in dengue suspected patients in Sri-Lanka

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dc.contributor.author Ngwe, T.M.M.
dc.contributor.author Raini, S.K.
dc.contributor.author Fernando, L.
dc.contributor.author Gunawardene, Y.I.N.S.
dc.contributor.author Inoue, S.
dc.contributor.author Takamatsu, Y.
dc.contributor.author Urano, T.
dc.contributor.author Muthugala, R.
dc.contributor.author Hapugoda, M.
dc.contributor.author Morita, K.
dc.date.accessioned 2023-08-02T04:15:56Z
dc.date.available 2023-08-02T04:15:56Z
dc.date.issued 2023
dc.identifier.citation Journal of Infection and Public Health.2023;16(9):1435-1442. en_US
dc.identifier.issn 1876-0341
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/26472
dc.description indexed for MEDLINE en_US
dc.description.abstract Background: Zika Virus (ZIKV) is a re-emerging, arthropod-borne flavivirus transmitted by Aedes mosquitoes (Ae. aegypti and Ae. albopictus). The coexistence of dengue virus (DENV) and ZIKV concurrently has been associated with a wide array of neurological complications, which may influence the clinical outcomes of infections. Sri Lanka witnessed a severe dengue epidemic in 2017, characterized by extraordinary and severe disease manifestations with considerable morbidity. Therefore, this study assessed the potential occurrence of ZIKV infection during DENV outbreak in Sri Lanka from 2017 to 2019, which could bear substantial implications for public health. Methods: Five hundred ninety-five serum samples were procured from individuals suspected of dengue and admitted to Kandy National Hospital between 2017 and 2018 and the Negombo District General Hospital between 2018 and 2019. These samples underwent quantitative real-time RT-PCR (qRT-PCR) to identify the presence of the ZIKV gene, while enzyme-linked immunosorbent assay was employed to detect ZIKV-specific IgM and IgG antibodies. Focus reduction neutralization tests were subsequently conducted to confirm ZIKV infection. Results: Among the 595 serum samples, 6 (1.0%) tested positive for ZIKV using qRT-PCR. Anti-ZIKV IgM and IgG were identified in 18.0% and 38.6% patients. Sixty-six (11.0%) samples demonstrated the presence of anti-ZIKV IgM and IgG. Within ZIKV IgM-positive samples, 2.2% exhibited neutralizing antibodies against ZIKV. Through the implementation of qRT-PCR, ZIKV IgM detection, and neutralization testing, 2% and 3.7% cases of ZIKV infections were confirmed in the Kandy and Negombo regions, respectively. Conclusion: This study is the inaugural endeavor to substantiate the existence of ZIKV infection in Sri Lanka utilizing molecular and serological analysis. The findings of this investigation imply that ZIKV was circulating throughout the 2017-2019 DENV outbreak. These results underscore the necessity for improved preparedness for future outbreaks, fortifying governmental policies on public health, and establishing effective early warning systems regarding the emergence of these viruses en_US
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.subject Acute Zika virus infection en_US
dc.subject Dengue suspected en_US
dc.subject Molecular and serological detection en_US
dc.subject Patients en_US
dc.subject Sri Lanka en_US
dc.title Epidemiological evidence of acute transmission of zika virus infection in dengue suspected patients in Sri-Lanka en_US
dc.type Article en_US


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