Digital Repository

Genome organization, in-silico structure, and cellular localization of putative lipid transporter, ARV1 from parasitic nematode Setaria digitata

Show simple item record

dc.contributor.author Wickramatunga, P.G.T.S.
dc.contributor.author Gunawardene, Y.I.N.S.
dc.contributor.author Chandrasekharan, N.V.
dc.contributor.author Dassanayake, R.S.
dc.date.accessioned 2022-08-16T06:29:46Z
dc.date.available 2022-08-16T06:29:46Z
dc.date.issued 2022
dc.identifier.citation Gene Reports.2022;28:101643 en_US
dc.identifier.issn 24520144
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/25074
dc.description Index in Scopus en_US
dc.description.abstract Setaria digitata, a nematode that lives in the peritoneal cavity of ruminants is the causative agent of cerebrospinal nematodiasis affecting livestock health. The ‘ACAT related enzyme 2 required for viability 1’ (arv-1) gene encodes putative lipid transporter that is essential in eukaryotes. The molecular characterization of nematode arv-1 has scarcely been studied and putative arv-1 isolated from S. digitata was used for this purpose. Docking and computer simulation studies using a modeled 3D structure of S. digitata ARV1 (Sd-ARV-1) with ceramide ligands revealed that the amino acid residues, Ile182, Leu56, Ala61, Gln186 and Gln146 are likely involved in the formation of potential sphingolipid binding sites having the same conserved residues in other nematodes. Sd-arv-1, a single copy gene, genomic region (1676 bp) had five exons encoding 217 amino acids, being interspersed by four introns showing a similar gene organization to other nematodes. Sd-ARV-1 is expressed ubiquitously at all development stages of the S. digitata life cycle. Tissue localization analyses revealed that Sd-ARV-1 was significantly expressed in the longitudinal muscle layer, endodermis, uterine wall, eggs, growing embryos inside the uterus, microfilariae, intestinal wall, esophagus lumen, dorsal nerve cord and ventral nerve cord. Therefore, ARV1 is a structurally conserved, ubiquitously expressed protein, which may be involved in development, reproduction, tissue remodeling, muscle contraction etc., in nematodes. en_US
dc.language.iso en en_US
dc.publisher Elsevier Inc. en_US
dc.subject Conserved residues en_US
dc.subject Gene organization en_US
dc.title Genome organization, in-silico structure, and cellular localization of putative lipid transporter, ARV1 from parasitic nematode Setaria digitata en_US
dc.type Article en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Digital Repository


Browse

My Account