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ELISA based evaluation of antibody response to Leishmania in a region endemic for cutaneous leishmaniasis

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dc.contributor.author Piyasiri, S.B.
dc.contributor.author Samaranayake, T.N.
dc.contributor.author Silva, H.
dc.contributor.author Manamperi, N.H.
dc.contributor.author Karunaweera, N.D.
dc.date.accessioned 2022-07-26T07:06:39Z
dc.date.available 2022-07-26T07:06:39Z
dc.date.issued 2022
dc.identifier.citation Parasite Immunology.2022;44(9):e12940 [Epub 2022 Aug 3] en_US
dc.identifier.issn 0141-9838
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/24945
dc.description Indexed in MEDLINE. en_US
dc.description.abstract Aims: Leishmaniasis includes several clinical forms. While routine diagnosis of cutaneous leishmaniasis (CL) is by microscopy, an antibody response to CL has been reported in several recent studies. This study evaluated anti-leishmanial IgG antibody responses as a biomarker of active leishmaniasis and a measure of exposure to Leishmania. Methods and results: Sera from 50 untreated CL patients, 140 patients under treatment and 280 healthy individuals residing in endemic regions collected as part of an epidemiological survey, was analysed with an ELISA established in-house using receiver-operator-characteristic (ROC) curve at optimised cut-off value. The assay showed high performance as a diagnostic tool in identifying exposure in endemic individuals (sensitivity: 98%, specificity: 90.3%). All patients showed lower antibody levels over time since onset of lesion/s. Antibody levels were higher (p ˂ 0.01) and persisted for a longer period in untreated patients. In patients under treatment, the level of anti-IgG antibodies was negatively correlated with the total duration the patient had been on treatment. Conclusion: The anti-leishmanial IgG response in L. donovani induced CL is transient and is unlikely to confer protective immunity. Optimised serological assays may be useful in endemic settings for diagnosis and monitoring the treatment response in CL. en_US
dc.language.iso en en_US
dc.publisher Oxford en_US
dc.subject ELISA en_US
dc.subject IgG antibodies en_US
dc.title ELISA based evaluation of antibody response to Leishmania in a region endemic for cutaneous leishmaniasis en_US
dc.type Article en_US


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