Digital Repository

Antioxidant, anti-inflammatory and antibacterial potentials of aqueous leaf extract of Pouteria campechiana (Kunth) Baehni (Ratalawulu)

Show simple item record

dc.contributor.author Hettiarachchi, O. I.
dc.contributor.author Deraniyagala, S. A.
dc.contributor.author Hettiarachchi, C. M.
dc.date.accessioned 2020-11-24T09:19:14Z
dc.date.available 2020-11-24T09:19:14Z
dc.date.issued 2020
dc.identifier.citation Hettiarachchi, O. I., Deraniyagala, S. A. and Hettiarachchi, C. M. (2020). Antioxidant, anti-inflammatory and antibacterial potentials of aqueous leaf extract of Pouteria campechiana (Kunth) Baehni (Ratalawulu). In: International conference on Frontiers in Chemical Technology 2020. Institute of Chemistry Ceylon, p.20. en_US
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/21619
dc.description.abstract Antioxidants are of dietary importance in preventing oxidative stress due to the production of excess free radicals in our body leading to many diseases including cancer, atherosclerosis and diabetes as well in aging. Despite the advances in modern medicine, plant -based remedies play an important role in healthcare due to less side effects. Due to the side effects of NSAIDS and antibiotics, plant -based remedies would be beneficial. This study was conducted to assess the potential use of P. campechiana (Ratalawulu) as an antioxidant, anti-inflammatory and antibacterial agent. The aqueous leaf extract of Ratalawulu was prepared according to the method of decoction preparation in ayurvedic medicine. All assays were carried out according to previously published procedures. Aqueous leaf extract of P. Baehni showed a Total Phenolic Content of 230.71±7.62 mg (PGE)/g in the Folin-Ciocalteu method. Total flavonoid content was 140.98 ± 98.46 mg (QE)/g according to AlCl3 colorimetric assay [PGE- Pyrogallol equivalence, QE- Quercetin equivalence]. 2,2-Diphenyl-1-picrylhydrazyl radical scavenging activity of aqueous leaf extract of P. Baehni ranged between 1.4%-17.8% for concentration between 0.01-0.1 mg/ml whereas ascorbic acid showed 2.46%-43.93% for the same concentration range. Ferric ion reducing assay for aqueous leaf extract of P. Baehni showed gradual increase in reducing property relating to antioxidant action by donating a hydrogen atom to break free radical chains. Hydroxyl scavenging activity showed 5.73%-44.03% for concentration series of 20-100 mg/ ml whereas, Ascorbic acid ranged from 9.40%-47.06%. Hydroxyl radical is one of the potent reactive oxygen species in the biological system. Nitric oxide scavenging anti-inflammatory assay showed 58.06%-75.36% whereas ascorbic acid showed 80.73%-91.76% for concentration series of 0.4-6.4 mg/ ml. In the human red blood cell assay, percentage protection by aqueous leaf extract of P. Baehni was 15.0%-62.4% and that of ascorbic acid was 12.1%-72.8% for 6.0-300 μg/ml. Aqueous leaf extract of P. Baehni also showed antibacterial activity against Staphylococcus aureus. Taken together, these results suggest the potential use of aqueous leaf extract of P. Baehni as an antioxidant, anti-inflammatory and antibacterial agent, and merit further related investigation. en_US
dc.publisher Institute of Chemistry Ceylon Adamantane House, Rajagiriya, Sri Lanka. en_US
dc.subject Ratalawulu, Antioxidant, Anti-inflammatory, Antibacterial en_US
dc.title Antioxidant, anti-inflammatory and antibacterial potentials of aqueous leaf extract of Pouteria campechiana (Kunth) Baehni (Ratalawulu) en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search Digital Repository


Browse

My Account