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In situ immune response to cutaneous leishmaniasis in Sri Lanka

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dc.contributor.author Manamperi, N.H.
dc.contributor.author Oghumu, S.
dc.contributor.author Pathirana, N.
dc.contributor.author de Silva, M.V.C.
dc.contributor.author Abeyewickreme, W.
dc.contributor.author Satoskar, A.R.
dc.contributor.author Karunaweera, N.D.
dc.date.accessioned 2017-10-24T03:59:19Z
dc.date.available 2017-10-24T03:59:19Z
dc.date.issued 2017
dc.identifier.citation Sri Lanka Medical Association, 130th Anniversary International Medical Congress. 2017;62(Supplement 1):191 en_US
dc.identifier.issn 0009-0895
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/17863
dc.description Poster Presentation Abstract (PP 091), 130th Anniversary International Medical Congress, Sri Lanka Medical Association, 13th-16th July 2017 Colombo, Sri Lanka en_US
dc.description.abstract INTRODUCTION & OBJECTIVES: Cutaneous leishmaniasis (CL) in Sri Lanka is caused by Leishmania donovani-MON 37, known to cause visceral leishmaniasis elsewhere. Localized immune response may play a role in disease outcome with T helper (Th) 1 response favouring lesion healing and Th2 response leading to disease progression in animal models. This study describes the localized host immune response to CL in Sri Lanka. METHOD: Skin punch biopsies from 58 patients with parasitologically confirmed CL and 25 healthy controls were quantified for cytokine gene expression of Th1 cytokines interferon (IFN)-γ, interleukin (IL)-12A and tumour necrosis factor (TNF)-α and Th2 cytokines, IL-4 and IL-10 by real-time RT-PCR. Relative copy numbers were calculated using the 2-ΔΔCt method. Non-parametric Mann-Whitney U test and the Spearman’s correlation test were used for statistical analysis. RESULTS: Study group consisted of 37 (63.8%) males and 21 (36.2%) females with a mean age of 35.0 years (SD=12.1, range=18-66), mean lesion duration of 6.75 ±9.1 months (range: 1-48) and a mean size of 176.59±185.76 mm2 (range: 12.6–908.3 mm2). Significant up regulation of IFN-γ (p<0.001) and down regulation of IL-4 (p<0.001) were seen in patients compared to healthy controls. Time taken for lesions to heal correlated significantly with in situ expression of IL-4 (Spearman’s r=0.321, p=0.034). CONCLUSION: Immune response to L. donovani induced CL in Sri Lanka tends to follow the typical Th1/Th2 convention with a Th2 biased milieu favouring poor responsiveness to antimony and delayed lesion healing. en_US
dc.language.iso en_US en_US
dc.publisher Sri Lanka Medical Association en_US
dc.subject Cutaneous leishmaniasis en_US
dc.title In situ immune response to cutaneous leishmaniasis in Sri Lanka en_US
dc.type Conference Abstract en_US


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    Papers presented at local and international conferences by the Staff of the Faculty of Medicine

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