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Single antigen detects both immunoglobulin M (IgM) and IgG antibodies elicited by all four dengue virus serotypes

Show simple item record Hapugoda, M.D. en_US Batra, G. en_US Abeyewickreme, W. en_US Swaminathan, S. en_US Khanna, N. en_US 2014-10-29T09:24:58Z 2014-10-29T09:24:58Z 2007 en_US
dc.identifier.citation Clinical and Vaccine Immunology. 2007; 14(11): pp.1505-14 en_US
dc.identifier.issn 1556-6811 (Print) en_US
dc.identifier.issn 1556-679X (Electronic) en_US
dc.description Indexed in MEDLINE
dc.description.abstract The resurgence of dengue (DEN) virus infections in the last few decades coupled with the lack of a preventive vaccine and specific antiviral drugs has jointly contributed to making this a significant global public health problem. Currently, symptomatic supportive treatment and fluid replacement therapy are the only means available to minimize DEN-induced mortality. As the clinical symptoms associated with DEN virus infections are indistinguishable from those of many other viral, bacterial, and parasitic infections, specific diagnostic tests assume critical importance in the unequivocal identification of DEN virus infections. We have designed a novel chimeric antigen based on envelope domain III (EDIII), a critical antigenic region of the major structural protein of DEN viruses. We fused EDIIIs corresponding to each of the four DEN virus serotypes using pentaglycyl linkers, over expressed the resultant tetravalent chimeric protein in Escherichia coli, and affinity purified it in high yields, obtaining approximately 30 mg protein of >95% purity per liter of culture. We show that this tetravalent antigen could specifically recognize anti-DEN virus antibodies of both the immunoglobulin M (IgM) and IgG classes. Using a large panel of IgM antibody capture-enzyme-linked immunosorbent assay- and hemagglutination inhibition-confirmed DEN virus-infected and uninfected patient sera (n = 289), we demonstrate that this tetravalent antigen can function as a diagnostic tool of high sensitivity and specificity.
dc.publisher American Society for Microbiology en_US
dc.title Single antigen detects both immunoglobulin M (IgM) and IgG antibodies elicited by all four dengue virus serotypes en_US
dc.type Article en_US
dc.identifier.department MMU en_US
dc.identifier.department Parasitology en_US
dc.creator.corporateauthor American Society for Microbiology en_US

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