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Isolation and subgrouping of rotaviruses from buffalo calves in Sri Lanka

Show simple item record Sunil-Chandra, N.P. Mahalingam, S. 2017-09-14T10:55:02Z 2017-09-14T10:55:02Z 1996
dc.identifier.citation Research in Veterinary Science. 1996; 60(2): 187-9. en_US
dc.identifier.issn 0034-5288 (Print)
dc.identifier.issn 1532-2661 (Electronic)
dc.identifier.issn 0034-5288 (Linking)
dc.description Indexed in MEDLINE en_US
dc.description.abstract Twenty-eight faecal specimens from Sri Lankan buffalo calves shown to be positive for rotavirus group A antigen were subgrouped by an enzyme-linked immunosorbent assay, by using monoclonal antibodies prepared against subgroup I and II antigens. The 13 of the 28 specimens which were classified as strongly positive belonged to subgroup I. Three of five faecal specimens inoculated on to roller cultures of MA104 cells yielded group A subgroup I rotavirus. As with other group A rotaviruses isolated from human beings and young animals, the buffalo isolates required pre-treatment with trypsin and to have trypsin incorporated in the maintenance medium, and the inoculated cell cultures had to be rolled; at least six serial passages were required before distinct rotavirus cytopathic effects were produced in the MA104 cells. en_US
dc.language.iso en_US en_US
dc.publisher British Veterinary Association, Elsevier en_US
dc.subject Buffaloes en_US
dc.subject Rotavirus en_US
dc.subject Rotavirus-classification en_US
dc.subject Rotavirus-isolation & purification en_US
dc.subject Rotavirus Infections en_US
dc.subject Rotavirus Infections-veterinary en_US
dc.subject Antigens, Viral en_US
dc.title Isolation and subgrouping of rotaviruses from buffalo calves in Sri Lanka en_US
dc.type Article en_US

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