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A Double antibody sandwich ELISA for the diagnosis of vivax malaria: a tool for further research

Show simple item record Seneviratna, G.D.C.N. en_US Manamperi, A.A.P.S. en_US Kapilananda, G.M.G. en_US Longacre, S. en_US Handunnetti, S.M. en_US Udagama-Randeniya, P.V. en_US 2014-10-29T09:18:39Z 2014-10-29T09:18:39Z 2000 en_US
dc.identifier.citation Ceylon Journal of Medical Science. 2000; 43(1): pp.11-17 en_US
dc.identifier.issn 0011-2232 en_US
dc.description.abstract A diagnostic assay for Plasmodium vivax based on a double antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA) was established to detect the merozoite surface protein 1 (PvMSP1) in wild isolates. This assay was based on the recombinant protein p19, a C-terminal processing product of PvMSP1. Of the two anti-P. vivax monoclonal antibodies (MAbs) selected, A21 was used as the capture antibody while horse radish peroxidase labelled A8 served as the probing second antibody. Optimized conditions established for p19 based DAS ELISA with the exception of a lower concentration of Tween-20 in buffers were suitable to screen lysed whole blood of malaria patients. This assay had a specificity of 100 percent for P. vivax and all the isolates of P. falciparum tested negative. Of the P. vivax-infected blood samples, only those containing both compact and schizont stages were diagnosed positive. The rest of the isolates tested negative either due to stage specificity of this assay or to the antigenic diversity of PvMSP1 in wild isolates. This test demonstrated a sensitivity of 27.58 percent and an accuracy of 63.15 percent. The positive and negative predicted values of this ELISA were 100 percent and 57.14 percent, respectively. Therefore, the P. vivax specific DAS ELISA developed and tested in the present study is not sufficiently sensitive to be used as a diagnostic tool for vivax malaria. Nevertheless, a baseline was established for development of diagnostic ELISA for future use with a more appropriate antigen. en_US
dc.publisher University of Colombo en_US
dc.subject Malaria en_US
dc.title A Double antibody sandwich ELISA for the diagnosis of vivax malaria: a tool for further research en_US
dc.type Article en_US
dc.identifier.department Parasitology en_US

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