Digital Repository

A Simple and rapid non-radioactive oligonucleotide based hybridization assay for the detection of Wuchereria bancrofti.

Show simple item record

dc.contributor.author Gunawardene, Y.I.N.S. en_US
dc.contributor.author Wijesundera, W.S. en_US
dc.contributor.author Karunanayake, E.H. en_US
dc.contributor.author Chandrasekharan, N.V. en_US
dc.contributor.author Jayasekara, N. en_US
dc.contributor.author Siridewa, K. en_US
dc.date.accessioned 2014-10-29T09:18:21Z
dc.date.available 2014-10-29T09:18:21Z
dc.date.issued 1999 en_US
dc.identifier.citation The Southeast Asian Journal of Tropical Medicine and Public Health. 1999; 30(2): pp.350-55 en_US
dc.identifier.uri http://www.tm.mahidol.ac.th/seameo/1999-30-2/1999-30-2-350.pdf
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/1400
dc.description Indexed in MEDLINE
dc.description.abstract Five biotin labeled oligonucleotides was designed based on a previously cloned and characterized repetitive DNA sequence specific for Wuchereria bancrofti. The oligonucleotide mix (containing five probes) when used in a hybridization assay, detected as little as 100 pg of purified W. bancrofti, microfilarial DNA, a single infective stage larva and a single microfilaria in 50 microl blood sample. A simple protocol was followed for the hybridization assay. Blood samples lysed with sterile distilled water and digested with proteinase K in the presence of a detergent were directly applied on to nylon membranes for dot blot assays. The DNA extract of mosquitos carrying infective stage larvae was eluted through sephadex G-50 minicolumns prior to blotting. The assay was also able to detect DNA extracted from microfilariae infected samples stored over five days at room temperature (28 degrees C). This simple and rapid oligonucleotide hybridization protocol with the highly sensitive chemiluminescent-based detection has significant potential for the development of a field kit to detect W. bancrofti infection.
dc.publisher SEAMEO Regional Tropical Medicine and Public Health Project en_US
dc.subject Filariasis
dc.subject Elephantiasis, Filarial-diagnosis
dc.subject Oligonucleotide Probes
dc.subject DNA, Helminth-analysis
dc.subject Wuchereria bancrofti-isolation and purification
dc.title A Simple and rapid non-radioactive oligonucleotide based hybridization assay for the detection of Wuchereria bancrofti. en_US
dc.type Article en_US
dc.identifier.department Parasitology en_US
dc.creator.corporateauthor SEAMEO Regional Tropical Medicine and Public Health Project en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Digital Repository


Browse

My Account