Browsing Journal/Magazine Articles by Author "Gunawardene, Y.I.N.S."

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Absence of Wolbachia endobacteria in Sri Lankan isolates of the nematode parasite of animals Setaria digitata

Voronin, D.; Abeykoon, A.M.; Gunawardene, Y.I.N.S.; Dassanayake, R.S. (Elsevier, 2015)

Setaria digitata is an animal filarial parasite with natural hosts of cattle and buffaloes that causes mild disease conditions. Infection of non-permissive hosts such as goats, sheep and horses, by this nematode can cause ...
An ARV1 homologue from a filarial nematode is functional in yeast

Herath, H.M.L.P.B.; Gunawardene, Y.I.N.S.; Pathiranage, M.; Wickramasinghe, P.D.S.U.; Wickramatunga, P.G.T.S.; Dassanayake, R.S. (London School Of Hygiene And Tropical Medicine, 2019)

The transmembrane protein, ARV1, plays a key role in intracellular sterol homeostasis by controlling sterol distribution and cellular uptake. To date, only the ARV1s from yeast and humans have been characterized to some ...
Assessment of developmental and reproductive fitness of dengue-resistant transgenic Aedes aegypti and Improvement of fitness using antibiotics

Ramyasoma, H.P.B.K.D.; Gunawardene, Y.I.N.S.; Hapugoda, M.; Dassanayake, R.S. (Hindawi Pub. Co., 2021)

BACKGROUND: Genetic modification offers opportunities to introduce artificially created molecular defence mechanisms to vector mosquitoes to counter diseases causing pathogens such as the dengue virus, malaria parasite, ...
Characterization of a novel cellular retinoic acid/retinol binding protein from shrimp: expression of the recombinant protein for immunohistochemical detection and binding assay

Gu, P.L.; Gunawardene, Y.I.N.S.; Chow, B.C.; He, J.G.; Chan, S.M. (Elsevier/North-Holland, 2002)

Members of the cellular retinoic acid (CRABP) and retinol binding (CRBP) proteins family are involved in the metabolic pathways of retinoic acid (RA) and retinal respectively. The objective of this study is to determine ...
Comparative immunohistochemistry and cellular distribution of farnesoic acid o-methyltransferase in the shrimp and the crayfish

Gunawardene, Y.I.N.S.; Bendena, W.G.; Tobe, S.S.; Chan, S.M. (Elsevier, 2003)

Farnesoic acid O-methyltransferase (FAMeT) catalyzes the conversion of farnesoic acid (FA) to methylfarnesoate (MF) by the mandibular organ (MO) of crustaceans. Here we report the cellular localization of FAMeT and ...
Could quantitative real-time polymerase chain reaction assay serve as an alternative test method to evaluate human epidermal growth factor receptor 2 status of gastric carcinoma in the South Asian setting?

Kannangara, D.K.S.; Lokuhetty, M.D.S.; Subasinghe, D.; Gunawardene, Y.I.N.S.; Dassanayake, R.S. (Indian Society of Gastroenterology/Springer India, 2019)

BACKGROUND:Human epidermal growth factor receptor 2 (HER2) protein overexpression and/or HER2 gene amplification are/is linked to a dismal outcome of gastric carcinoma (GCa). Immunohistochemistry (IHC) and fluorescence in ...
Development of single-strand conformational polymorphism to screen for mutations in hotspot regions of beta-globin gene of beta-thalassemia patients of Sri Lanka

Dassanayake, R.S.; Mahadevan, K.; Gunawardene, Y.I.N.S. (SEAMEO Regional Tropical Medicine and Public Health Project, 2013)

Beta-thalassemia is prevalent in Sri Lanka and imposes a heavy economic and social burden in the country due to the patients' life-long need for regular blood transfusion and treatment with iron chelation therapy. Thus, ...
Development of siRNA mediated RNA interference and functional analysis of novel parasitic nematode-specific protein of Setaria digitata

Somarathne, M.B.C.L.; Gunawardene, Y.I.N.S.; Chandrasekharan, N.V.; Dassanayake, R.S. (Academic Press, 2018)

Despite the differences of the host, parasitic nematodes may share commonalities in their parasitizing genes. Setaria digitata novel protein (SDNP) is such an entity which is parasitic nematode-specific and having sequence ...
Development of the sterile Insect technique to control the dengue vector Aedes aegypti (Linnaeus) in Sri Lanka

Ranathunge, T.; Harishchandra, J.; Maiga, H.; Bouyer, J.; Gunawardene, Y.I.N.S.; Hapugoda, M. (Public Library of Science,San Francisco, 2022)

Background: The Sterile Insect Technique (SIT) is presently being tested to control dengue in several countries. SIT aims to cause the decline of the target insect population through the release of a sufficient number of ...
Entomological surveillance with viral tracking demonstrates a migrated viral strain caused dengue epidemic in July, 2017 in Sri Lanka.

Withanage, G.P.; Hapuarachchi, H.C.; Viswakula, S.D.; Gunawardene, Y.I.N.S.; Hapugoda, M. (Public Library of Science, 2020)

BACKGROUND: Dengue is the most important mosquito-borne viral infection disease in Sri Lanka triggering extensive economic and social burden in the country. Even after numerous source reduction programmes, more than 30,000 ...
Epidemiological evidence of acute transmission of zika virus infection in dengue suspected patients in Sri-Lanka

Ngwe, T.M.M.; Raini, S.K.; Fernando, L.; Gunawardene, Y.I.N.S.; Inoue, S.; Takamatsu, Y.; Urano, T.; Muthugala, R.; Hapugoda, M.; Morita, K. (Elsevier, 2023)

Background: Zika Virus (ZIKV) is a re-emerging, arthropod-borne flavivirus transmitted by Aedes mosquitoes (Ae. aegypti and Ae. albopictus). The coexistence of dengue virus (DENV) and ZIKV concurrently has been associated ...
Evaluating novel effective primers to amplify heterozygous alleles of second, third and fourth exons of HLA-A;-B;-C;-DRB1 and-DQB1 loci using sequencing-based typing.

Perera, P.C.D.; Upamali, B.D.N.; Gunawardene, Y.I.N.S.; Dassanayake, R.S. (National Science Foundation, 2019)

ABSTRACT: Human leukocyte antigen (HLA) typing is one of the most crucial steps that determines the success of an organ transplant. However, HLA typing is a challenging task due to the diversity of HLA alleles, which is ...
Evaluation of the effects of Aedes vector indices and climatic factors on dengueincidence in Gampaha District, Sri Lanka

Wijegunawardana, N.D.A.D.; Gunawardene, Y.I.N.S.; Chandrasena, T.G.A.N.; Dassanayake, R.S.; Udayanga, N.W.B.A.L.; Abeyewickreme, W. (Hindawi Publishing Corporation, 2019)

Constant monitoring of Aedes vector indices such as Aedes mosquito abundance and ovitrap data is important for the control of dengue epidemics. Therefore, the current study attempted to evaluate the effect of larval and ...
Evolutionary selective trends of insect/mosquito antimicrobial defensin peptides containing cysteine-stabilized alpha/beta motifs

Dassanayake, R.S.; Gunawardene, Y.I.N.S.; Tobe, S.S. (Elsevier, 2007)

Insect defensins containing cysteine-stabilized alpha/beta motifs (Cs-alpha/beta defensin) are cationic, inducible antibacterial peptides involved in humoral defence against pathogens. To examine trends in molecular evolution ...
Function and cellular localization of farnesoic acid o-methyltransferase (FAMeT) in the shrimp, Metapenaeus ensis

Gunawardene, Y.I.N.S.; Tobe, S.S.; Bendena, W.G.; Chow, B.K.C; Yagi, K.J.; Chan, S.M. (Wiley-Blackwell, 2002)

The isoprenoid methyl farnesoate (MF) has been implicated in the regulation of crustacean development and reproduction in conjunction with eyestalk molt inhibiting hormones and ecdysteroids. Farnesoic acid O-methyltransferase ...
Functional analysis of a novel parasitic nematode-specific protein of Setaria digitata larvae in Culex quinquefasciatus by siRNA mediated RNA interference

Somarathne, M.B.C.L.; Gunawardene, Y.I.N.S.; Chandrasekharan, N.V.; Ellepola, A.N.B.; Dassanayake, R.S. (BioMed Central, 2018)

BACKGROUND: Functional analysis of animal parasitic nematode genes is often quite challenging due to the unavailability of standardised in vitro culture conditions and lack of adequate tools to manipulate these genes. ...
Geneder differences in undergraduate medical examination results in Sri Lanka

de Silva, N.R.; Thabrew, M.I.; Saparamadu, P.A.M.; Jayawardena, D.R.K.C.; Arachchige, A.A.; Weerawardhane, M.; Gunawardene, Y.I.N.S. (Sri Lanka Medical Association, 2000)

OBJECTIVE: To identify possible gender related differences in performance at undergraduate medical examinations in Sri Lanka. STUDY DESIGN AND METHODS: Results of examinations conducted by the faculty of medicine, University ...
Genome organization, in-silico structure, and cellular localization of putative lipid transporter, ARV1 from parasitic nematode Setaria digitata

Wickramatunga, P.G.T.S.; Gunawardene, Y.I.N.S.; Chandrasekharan, N.V.; Dassanayake, R.S. (Elsevier Inc., 2022)

Setaria digitata, a nematode that lives in the peritoneal cavity of ruminants is the causative agent of cerebrospinal nematodiasis affecting livestock health. The ‘ACAT related enzyme 2 required for viability 1’ (arv-1) ...
Geographic information system (GIS) mapping of lymphatic filariasis endemic areas of Gampaha District, Sri Lanka based on epidemiological and entomological screening

Wijegunawardana, N.D.A.D.; Gunawardene, Y.I.N.S.; Manamperi, A.; Senarathne, H.; Abeyewickreme, W. (SEAMEO Regional Tropical Medicine and Public Health Project, 2012)

The objective of this study was to develop a site directed geographic information system (GIS) map of lymphatic filariasis (LF) in Gampaha District, Sri Lanka as a guide for targeted control activities. Epidemiological and ...
Heterologous expression, chaperone mediated solubilization and purification of parasitic nematode-specific growth factor-like protein of Setaria digitata

Rodrigo, W.W.P.; Dassanayake, R.S.; Karunanayake, E.H.; Gunawardene, Y.I.N.S.; Weerasena, O.V.D.S.J. (Asian Pacific Tropical Medicine Press , China, 2014)

OBJECTIVE: To clone, express and purify a putative parasitic nematode specific protein of Setaria digitata (S. digitata), filarial nematode that infects livestock and cause significant economic losses in Far East and Asia ...

Browsing Journal/Magazine Articles by Author "Gunawardene, Y.I.N.S."

Browsing Journal/Magazine Articles by Author "Gunawardene, Y.I.N.S."

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