Browsing Journal/Magazine Articles by Author "Chandrasekharan, N.V."

Now showing items 1-11 of 11

Application of a real time Polymerase Chain Reaction (PCR) assay for the early diagnosis of human leptospirosis in Sri Lanka

Denipitiya, D.T.H.; Chandrasekharan, N.V.; Abeyewickreme, W.; Hartskeerl, C.M.; Hartskeerl, R.A.; Jiffrey, A.M.; Hapugoda, M.D. (Academic Press, Elsevier, 2016)

Leptospirosis has a major impact on health in Sri Lanka but is probably grossly under-recognized due to difficulties in clinical diagnosis and lack of diagnostic laboratory services. The objective of this study was to ...
Cloning and characterization of a repetitive DNA sequence specific for Wuchereria bancrofti

Siridewa, K.; Karunanayake, E.H.; Chandrasekharan, N.V.; Abeyewickreme, W.; Franzen, L.; Aslund, L.; Pettersson, U. (American Society of Tropical Medicine and Hygiene, 1994)

A genomic library constructed in a bacteriophage lambda replacement vector (EMBL3) with Wuchereria bancrofti DNA partially digested with Sau 3A I was screened with 32P-labeled W. bancrofti total DNA, and a strongly reactive ...
Development of a low cost semiquantitative polymerase chain reaction assay for molecular diagnosis of williams syndrome

Ranaweera, D.M.; de Silva, D.C.; Samarasinghe, D.; Perera, S.; Kugalingam, N.; Samarasinghe, S.R.; Madushani, W.Y.; Jayaweera, H.H.E.; Gunewardene, S.; Muneeswaran, K.; Gnanam, V.S.; Chandrasekharan, N.V. (Clinical Laboratory Publications, 2024)

BACKGROUND: Williams Beuren Syndrome (WBS) is a well-recognized and common genetic cause of congenital heart defects, developmental delay, hypercalcemia, and characteristic facial features. It is caused by a 1.5 - 1.8 Mb ...
Development of siRNA mediated RNA interference and functional analysis of novel parasitic nematode-specific protein of Setaria digitata

Somarathne, M.B.C.L.; Gunawardene, Y.I.N.S.; Chandrasekharan, N.V.; Dassanayake, R.S. (Academic Press, 2018)

Despite the differences of the host, parasitic nematodes may share commonalities in their parasitizing genes. Setaria digitata novel protein (SDNP) is such an entity which is parasitic nematode-specific and having sequence ...
Dirofilaria repens: Cloning and Characterization of a Repeated DNA Sequence for the Diagnosis of Dirofilariasis in Dogs, Canis familiaris

Chandrasekharan, N.V.; Karunanayake, E.H.; Franzen, L.; Abeyewickreme, W.; Pettersson, U. (Academic Press, 1994)

A highly repetitive DNA element from the genome of the filarial nematode Dirofilaria repens has been cloned and sequenced. The 176-base pair repeating units are arranged in direct tandem and are clustered in the parasite ...
Evaluation of 22q11.2 deletion in Cleft Palate patients.

Prabodha, L.B.; Dias, D.K.; Nanayakkara, B.G.; de Silva, D.C.; Chandrasekharan, N.V.; Ileyperuma, I. (Mumbai : Medknow Publications, 2012)

BACKGROUND: Cleft palate is the commonest multifactorial epigenetic disorder with a prevalence of 0.43-2.45 per 1000. The objectives of this study were to evaluate the clinical features and identify the 22q11.2 deletion ...
Functional analysis of a novel parasitic nematode-specific protein of Setaria digitata larvae in Culex quinquefasciatus by siRNA mediated RNA interference

Somarathne, M.B.C.L.; Gunawardene, Y.I.N.S.; Chandrasekharan, N.V.; Ellepola, A.N.B.; Dassanayake, R.S. (BioMed Central, 2018)

BACKGROUND: Functional analysis of animal parasitic nematode genes is often quite challenging due to the unavailability of standardised in vitro culture conditions and lack of adequate tools to manipulate these genes. ...
Genome organization, in-silico structure, and cellular localization of putative lipid transporter, ARV1 from parasitic nematode Setaria digitata

Wickramatunga, P.G.T.S.; Gunawardene, Y.I.N.S.; Chandrasekharan, N.V.; Dassanayake, R.S. (Elsevier Inc., 2022)

Setaria digitata, a nematode that lives in the peritoneal cavity of ruminants is the causative agent of cerebrospinal nematodiasis affecting livestock health. The ‘ACAT related enzyme 2 required for viability 1’ (arv-1) ...
Identification of cattle, buffaloes and rodents as reservoir animals of Leptospira in the District of Gampaha, Sri Lanka

Denipitiya, D.T.H.; Chandrasekharan, N.V.; Abeyewickreme, W.; Hartskeerl, R.A.; Hapugoda, M.D. (Biomed Central, 2017)

BACKGROUND: Leptospirosis is an important emerging infectious disease in Sri Lanka. Rats are the most important reservoir of Leptospira but domestic and wild mammals may also act as important maintenance or accidental ...
Modified mismatch polymerase chain reaction-restriction fragment length polymorphism detected mutations in codon 12 and 13 of exon 2 of K-ras gene in colorectal cancer patients and its association with liver metastases: Data from a South Asian country

Faleel, F.D.; Zoysa, M.I.; Lokuhetti, M.D.; Gunawardene, Y.I.N.S.; Chandrasekharan, N.V.; Dassanayake, R.S. (Medknow Publications, 2016)

AIM: Mutations in K-ras codon 12 and 13 of exon 2 are known to affect prognosis and impart resistance to anti-epidermal growth factor monoclonal antibody therapy in colorectal carcinoma (CRC). Our aim was to investigate ...
A Simple and rapid non-radioactive oligonucleotide based hybridization assay for the detection of Wuchereria bancrofti.

Gunawardene, Y.I.N.S.; Wijesundera, W.S.; Karunanayake, E.H.; Chandrasekharan, N.V.; Jayasekara, N.; Siridewa, K. (SEAMEO Regional Tropical Medicine and Public Health Project, 1999)

Five biotin labeled oligonucleotides was designed based on a previously cloned and characterized repetitive DNA sequence specific for Wuchereria bancrofti. The oligonucleotide mix (containing five probes) when used in a ...

Browsing Journal/Magazine Articles by Author "Chandrasekharan, N.V."

Browsing Journal/Magazine Articles by Author "Chandrasekharan, N.V."

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