dc.contributor.author |
Siridewa, K. |
en_US |
dc.contributor.author |
Karunanayake, E.H. |
en_US |
dc.contributor.author |
Chandrasekharan, N.V. |
en_US |
dc.contributor.author |
Abeyewickreme, W. |
en_US |
dc.contributor.author |
Franzen, L. |
en_US |
dc.contributor.author |
Aslund, L. |
en_US |
dc.contributor.author |
Pettersson, U. |
en_US |
dc.date.accessioned |
2014-10-29T09:11:41Z |
|
dc.date.available |
2014-10-29T09:11:41Z |
|
dc.date.issued |
1994 |
en_US |
dc.identifier.citation |
The American Journal of Tropical Medicine and Hygiene. 1994; 51(4): pp.495-500 |
en_US |
dc.identifier.issn |
0002-9637 (Print) |
en_US |
dc.identifier.issn |
1476-1645 (Electronic) |
en_US |
dc.identifier.uri |
http://repository.kln.ac.lk/handle/123456789/1228 |
|
dc.description |
Indexed in MEDLINE |
|
dc.description.abstract |
A genomic library constructed in a bacteriophage lambda replacement vector (EMBL3) with Wuchereria bancrofti DNA partially digested with Sau 3A I was screened with 32P-labeled W. bancrofti total DNA, and a strongly reactive recombinant, EMBL3Wb34, was isolated. This clone contained an approximately 16-kb insert that showed some cross-hybridization with Brugia malayi and B. pahangi DNA. However, a 969-bp subclone from EMBL3Wb34, designated pWb12, hybridized only with W. bancrofti DNA and was able to detect as little as 300 pg. Furthermore, pWb12 could detect DNA from a single infective larva or one microfilaria. It has a moderate copy number (450-700) and appears to be interspersed within the parasite genome. The nucleotide sequence contains 66% A+T and 34% G+C and shows no notable internal repeats. |
en_US |
dc.publisher |
American Society of Tropical Medicine and Hygiene |
en_US |
dc.subject |
DNA, Helminth-chemistry |
en_US |
dc.subject |
Repetitive Sequences, Nucleic Acid |
en_US |
dc.subject |
Wuchereria bancrofti-genetics |
en_US |
dc.subject |
Wuchereria bancrofti |
|
dc.title |
Cloning and characterization of a repetitive DNA sequence specific for Wuchereria bancrofti |
en_US |
dc.type |
Article |
en_US |
dc.identifier.department |
Parasitology |
en_US |
dc.creator.corporateauthor |
American Society of Tropical Medicine and Hygiene |
en_US |