Digital Repository

Crypt cell proliferation and HLA-DR expression in pelvic ileal pouches

Show simple item record de Silva, H.J. en_US Gatter, K.C. en_US Millard, P.R. en_US Kettlewell, M. en_US Mortensen, N.J. en_US Jewell, D.P. en_US 2014-10-29T09:10:07Z 2014-10-29T09:10:07Z 1990 en_US
dc.identifier.citation Journal of Clinical Pathology. 1990; 43(10): pp.824-828 en_US
dc.identifier.issn 0021-9746 (Print) en_US
dc.identifier.issn 1472-4146 (Electronic) en_US
dc.description Indexed in MEDLINE
dc.description.abstract To investigate the nature of the morphological changes that occur in ileal pouches, 26 biopsy specimens from patients with functioning ileo-anal pouches (eight with pouchitis) were studied. Normal ileum (n = 10) was used as a control. Mucosal morphometry (using linear measurements), crypt cell proliferation (CCP) (using the monoclonal antibody Ki67), and epithelial HLA-DR expression (monoclonal antibody CR3/43) were assessed. CCP (expressed as the percentage of Ki67 positive nuclei for each crypt) was significantly higher in pouches with pouchitis, compared with those without, and in pouches without pouchitis compared with normal ileum. CCP values in some pouches without pouchitis approached values found in those with pouchitis. CCP was related inversely to villous height and an index of villous atrophy (VH/TMT), and directly to crypt depth. In the presence of pouchitis there was intense epithelial HLA-DR expression that extended into the crypts. In some pouches with high CCP values, but without clinically important inflammation, surface epithelial HLA-DR expression was weak and patchy. It is concluded that villous atrophy and crypt hyperplasia in ileal pouches are associated with high CCP values. These may be increased even in the absence of active inflammation, and this increase may occur as a response to the new luminal environment. en_US
dc.publisher BMJ Publishing Group en_US
dc.subject Ileitis-pathology
dc.subject Ileum-immunology
dc.subject Ileum-pathology
dc.subject Ileum-surgery
dc.subject Immunoenzyme Techniques
dc.subject Colitis, Ulcerative-surgery
dc.subject Adenomatous Polyposis Coli-surgery
dc.subject Anal Canal-surgery
dc.subject Anastomosis, Surgical
dc.subject HLA-DR Antigens-immunology
dc.title Crypt cell proliferation and HLA-DR expression in pelvic ileal pouches en_US
dc.type Article en_US
dc.identifier.department Medicine en_US
dc.creator.corporateauthor Association of Clinical Pathologists (Great Britain) en_US
dc.creator.corporateauthor British Medical Association en_US

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Digital Repository


My Account