Medicine
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This repository contains the published and unpublished research of the Faculty of Medicine by the staff members of the faculty
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Item Clinical evaluation of rapid diagnostic test kit for scrub typhus with improved performance(Korean Academy of Medical Science, 2016) Kim, Y.J.; Park, S.; Premaratna, R.; Selvaraj, S.; Park, S.J.; Kim, S.; Kim, D.; Kim, M.S.; Shin, D.H.; Choi, K.C.; Kwon, S.H.; Seo, W.; Lee, N.T.; Kim, S.H.; Kang, H.K.; Kim, Y.W.Diagnosis of scrub typhus is challenging due to its more than twenty serotypes and the similar clinical symptoms with other acute febrile illnesses including leptospirosis, murine typhus and hemorrhagic fever with renal syndrome. Accuracy and rapidity of a diagnostic test to Orientia tsutsugamushi is an important step to diagnose this disease. To discriminate scrub typhus from other diseases, the improved ImmuneMed Scrub Typhus Rapid Diagnostic Test (RDT) was evaluated in Korea and Sri Lanka. The sensitivity at the base of each IgM and IgG indirect immunofluorescent assay (IFA) in Korean patients was 98.6% and 97.1%, and the specificity was 98.2% and 97.7% respectively. The sensitivity and specificity for retrospective diagnosis at the base of IFA in Sri Lanka was 92.1% and 96.1%. ImmuneMed RDT was not reactive to any serum from seventeen diseases including hemorrhagic fever with renal syndrome (n = 48), leptospirosis (n = 23), and murine typhus (n = 48). ImmuneMed RDT shows superior sensitivity (98.6% and 97.1%) compared with SD Bioline RDT (84.4% at IgM and 83.3% at IgG) in Korea. The retrospective diagnosis of ImmuneMed RDT exhibits 94.0% identity with enzyme-linked Immunosorbent assay (ELISA) using South India patient serum samples. These results suggest that this RDT can replace other diagnostic tests and is applicable for global diagnosis of scrub typhus. This rapid and accurate diagnosis will be beneficial for diagnosing and managing scrub typhusItem A Clinical and cost evaluation between outpatient endometrial biopsy and dilatation and curettage(Sri Lanka College of Obstetricians and Gynaecologists, 1997) Gunasekera, P.C.; Wijesinghe, P.S.; Saparamadu, P.A.M.Objective To evaluate the clinical and financial benefits of endometrial aspiration biopsy using a new aspiration device (Probet) over conventional dilatation and curettage. Design Comparison of histology and adequacy of samples obtained by endometrial aspiration and formal curettage. The histopathologist was blinded with regard to the biopsy method. Setting The gynaecology outpatient department of a teaching hospital. Patients 52 consecutive women requiring dilatation and curettage (D and C). Interventions Endometrial aspiration was performed in the out patient department and conventional D and C performed within 3 days. Main outcome measures Degree of discomfort to patients during endometrial aspiration was assessed by a visual analogue scale. Histological adequacy of the endometrial samples obtained was determined. The histological diagnosis of samples obtained by the two methods was passed. Cost of the two methods was determined. Results Eight patients experienced moderate to severe discomfort during endometrial aspiration. These were done for investigation of subfertility (2) and postmenopausal bleeding (6). Scanty or no endometrial aspirate was obtained in 9 women, 6 of whom had postmenopausal bleeding. No curettings were obtained in 5 of the women with postmenopausal bleeding. Active bleeding was present at the time of the procedure in 5 patients which was reported as blood clot and menstrual endometrium. In only 2 of the paired endometrial samples did the histological diagnosis differ from the specimen obtained by endometrial aspiration. Histological concordance was noted in 30 pairs. Conclusions Outpatient endometrial biopsy is acceptable to the majority of patients. Nulliparous and postmenopausal woman feel discomfort. Endometrial biopsy in the presence of active bleeding is of little value. There is good histological concordance between samples from endometrial aspiration is as ten times cheaper than conventional curettage