Medicine
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This repository contains the published and unpublished research of the Faculty of Medicine by the staff members of the faculty
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Item Evidence for an R(+)-[(dihydroindenyl)oxy]alkanoic acid-sensitive K+/Cl- co-transporter in human platelets and its interaction with the Na+/K+/2Cl- co-transporter.(Portland Press on behalf of the Medical Research Society and the Biochemical Society, 1997) de Silva, H.A.; Aronson, J.K.The K+/Cl- co-transport system is activated by a number of interventions, such as cell swelling and stimulation with N-ethylmaleimide. It is specifically inhibited by R(+)-[(dihydroindenyl)oxy]alkanoic acid and requires the presence of K+ and Cl- on the same side of the cell membrane. This co-transporter has been studied extensively, mainly in erythrocytes of many species, in which it plays a key role in cell volume regulation. Here we present evidence that human platelets contain K+/Cl- co-transporters. 2. We have studied the efflux of 86Rb+ (a marker for K+) from 86Rb(+)-loaded human platelets, and have defined their response to stimulation by N-ethylmaleimide. 3. N-Ethylmaleimide (0.5 and 1 mmol/l) stimulated an increase in cumulative 86Rb+ efflux in a concentration-dependent manner. This efflux was inhibited by R(+)-[(dihydroindenyl)oxy]alkanoic acid (10 mumol/l) but was insensitive to bumetanide. It also required the presence of external Cl-. 4. These observations suggest that 86Rb+ efflux from the platelets stimulated by N-ethylmaleimide occurs via K+/Cl- co-transport. 5. When the K+/Cl- co-transporter was stimulated by N-ethylmaleimide we were unable to stimulate the Na+/K+/2Cl- co-transporter with a high external concentration of KCl or inhibit 86Rb+ efflux with bumetanide. Together with other evidence, this suggests that when the K+/Cl- co-transporter is stimulated with N-ethylmaleimide, the Na+/K+/2Cl- co-transporter is inhibited.Item Does Aerua lanata have diuretic properties?(Dept. of Physiology, All-India Institute Of Medical Sciences, 1993) Goonaratna, C.; Thabrew, I.; Wijewardene, K.We have compared the diuretic activity of 200 ml of sucrose flavoured aqueous extract of the herb Aerua lanata with that of 200 ml sucrose flavoured weak infusion of tea leaves, and 200 ml of water, in 14 healthy volunteers under standardized conditions. Each volunteer acted as his or her own control in the three protocols, and chemical, physical and statistical analyses were performed "blind" by coding all urine and blood samples collected. A. lanata extract did not significantly increase urine flow, sodium excretion, potassium excretion, or urine and plasma osmolality as compared to an infusion of tea or plain water.