IPRC - 2015
Permanent URI for this collectionhttp://repository.kln.ac.lk/handle/123456789/156
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Item Selenium in rice consumed by Sri Lankans(Faculty of Graduate Studies, University of Kelaniya, 2015) Prasanna, E.G.J.; Wickramasingha, E.A.D.N.N.; de Silva, D.S.M.; Wimalasena, S.Selenium is an essential trace element found in the selenoproteins and as a component of enzymes such as glutathione peroxidase that could quench free radicals by acting as antioxidants and prevent free radical induced degenerative diseases such as diabetics, Alzheimer, rheumatism and the deficiency of selenium could result in iodine deficiency resulting goiter. It also proved that selenium is toxic in high concentrations resulting in damages to the nervous system and even some forms of cancer. Unlike most elements the essentiality and toxicity of selenium is shown in a narrow range the respective values being 40 μg day-1 and 400 μg day-1. Studies on selenium content in food have been reported from other countries but studies carried out in food consumed by Sri Lankans is rare. Hence, the objective of the present study is to determine the selenium content in different varieties of rice consumed by Sri Lankans. Parboiled rice is commonly consumed in Sri Lanka. Studies have not been carried out in Sri Lanka or any other country on the effect of parboiling on the selenium content. Hence, this study was extended to deduce the effect of parboiling on the selenium content in rice. Selenium content in fourteen varieties of rice namely Bg 250, Bg 352, Bg 358, Bg 359, Bg 360, Bg 379, Bg 450, Bg 094, Bw 361, Bw 363, Bw 364, Ld 408, At 306 and At 362 obtained from the Rice Research Institute, Batalagoda and subjected to acid digestion. Selenium contents of the samples were determined using Hydride Generation Atomic Absorption Spectrometric method. Experiments were carried out in triplicate. The selenium content (μg kg-1) in the rice samples analyzed ranged from 24.5 (At 362) - 40.5 (Bg 094). The selenium content in parboiled rice was analyzed by parboiling the above raw rice samples at home. The results of the present study showed that the selenium content (μg kg-1) in the grains from par boiled rice varied from 29.0 (Bw 361) to 42.0 (Bg 352). This study reveals that parboiling increased the selenium content in rice although a direct relation could not be obtained between the selenium content in raw rice and that in the parboiled rice.Item Optimization for Rock Phosphate Solubilization by Phosphate-Solubilizing Bacteria isolated from Oryza Sativa Rhizosphere(Faculty of Graduate Studies, University of Kelaniya, 2015) Gunawardana, M.B.D.S.S.; Ratnatilleke, A.A.L.Rock Phosphate (RP) is a cheap, phosphate-rich source but due to its lack of water solubility its use is limited in rice cultivation as a Phosphorus (P) fertilizer. However, certain bacteria in rice rhizosphere have shown the capability of solubilizing RP and supplying P to rice plants. Under certain conditions, the rate of releasing P could be low and these parameters should be optimized to obtain the full capacity of microorganisms as P biofertilizers. This study was undertaken to identify efficient RP solubilizing bacteria isolated from rice rhizosphere and the effects of parameters such as RP concentration, particle size, C:N ratio, chelation, mixed cultures, P source and Nitrogen source on their ability to solubilize RP. A pool of 165 naturally colonizing bacteria in rice plants isolated previously were screened for their RP solubilizing ability using solid and liquid modified PVK (Pikovskaya) selective media, containing RP as the sole P source. The observed highest P solubilizing strains, numbers 36 and 52, were tested along with a reference P- solubilizing strain, EP-11, which was isolated previously from Eppawala RP mines. The results showed that optimum RP concentration for strain 36 was 10.0g L-1 and 5.0g L-1 for strain 52. The content of soluble P gradually decreased with a larger particle size of RP with all the isolates. The highest content of soluble P was achieved when C:N ratio was 50:1, EDTA concentration was 2.0 mg ml-1, and Nitrogen source was NH4NO3 for both strains 36 and 52. However under the mixed cultures of strains 36 and 52, the solubilized P concentration was much lower (16.91 mg L-1) than their individual P solubilization levels. The optimization of these parameters would be important in achieving the maximum P solubility if strains 36 and 52 were to be developed in to P biofertilizers.