ICAPS 2024
Permanent URI for this collectionhttp://repository.kln.ac.lk/handle/123456789/28744
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Item Evaluation of antioxidant, antidiabetic properties and phytochemical composition of Impatiens repens stems(Faculty of Science, University of Kelaniya Sri Lanka, 2024) Kothalawala, N. K.; Abeykoon, A. M. D. M. B.Plant Impatiens repens has been used in Sri Lankan traditional medicine as a medicinal herbal plant to treat various diseases such as insanity, haemorrhoids and epilepsy. This plant is believed to contain a lot of phytochemicals which are responsible for antioxidant, antidiabetic and anti-inflammatory activities. Not only leaves, but stems, and roots are also related to certain medicinal properties which have been used for years as herbal medicines. In this research study, antioxidant, antidiabetic activities and phytochemical composition of stems of I. repens were evaluated. DPPH (2,2-diphenyl- 1-picrylhydrazyl) radical scavenging assay, total phenolic content (TPC) determination, total flavonoid content (TFC) determination and α-amylase inhibition assays were carried out in order to evaluate the antioxidant, antidiabetic activities and phytochemical composition of I. repens stems using solvents such as hexane, ethyl acetate and methanol. According to the results obtained for the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, the lowest IC50 value was obtained for methanol extract which is 3.536± 0.8416 mg/mL, with compared to hexane and ethyl acetate extracts. These results suggest that a significant antioxidant activity is present in the stems of I. repens. Total phenolic and flavonoid content of stems of I. repens were determined using TPC and TFC assays. According to the results obtained for total phenolic content, the highest proportion of phenolic content was obtained for ethyl acetate extract which is 173.157 ± 11.066 mg GAE /g extract while in total flavonoid content, the highest proportion of flavonoid content was obtained for methanol extract which is 44.623 ± 6.295 mg CE /g extract. Therefore, it can be concluded that a significant amount of phenolic and flavonoid content is present in the stems of I. repens. In α- amylase inhibitory assay, the lowest IC50 was obtained for the methanol extract with compared to hexane and ethyl acetate extracts which is 2.833 ± 0.760 mg/mL, indicating the highest inhibition percentage. Therefore, it can be concluded that a significant antidiabetic activity is present in the stems of I. repens, which is related to α- amylase enzyme inhibition. As a result of thin layer chromatography (TLC) plate preparation and gas chromatography (GC) analysis, a compound was identified which might be named as phenol,2,4-bis (1,1-dimethylethyl) in the ethyl acetate extract. This compound is determined as a compound which contains antioxidant and antimicrobial activities. Therefore, it can be concluded that the compound, phenol,2,4-bis-(1,1-dimethylethyl) might be present in the ethyl acetate extract and this compound might be responsible for the antioxidant and antimicrobial properties of stems of I. repens plant.