Abstract:
Plants belong to Violaceae family are known to contain stable bioactive cyclic peptides known as
cyclotides. Eight species of Violaceae plants can be found in Sri Lanka: three from genus Rinorea,
three from genus Viola and two from genus Hybanthus. Rinorea species were thought to be extinct
in Sri Lanka until the recent discovery of Rinorea decora and Rinorea bengalensis. However, the
chemical constituents and bioactivities of Sri Lankan Viola plants have not been investigated. Aim
of the study was to characterize cyclotides in Sri Lankan Viola plants using a complementary tool of
transcriptomics. Out of all Violaceae plants, V. betonicifolia was selected for transcriptome de novo
sequencing and analysis to identify cyclotide sequences. RNA from V. betonicifolia plant tissues were
extracted and sequenced by the platform Illumina HiSeq 2000 sequencing system with the library
TruSeq RNA. Analysis of precursor sequences and putative processing sites provides further insight
into cyclotide biosynthesis in V. betonicifolia and may also help to distinguish cyclotides from other
cysteine-rich plant proteins. A total of 28 new (vibe 1-28) and three known (kalata S, viba 17, viba 11)
cyclotide sequences were identified, which includes acyclotide and both cyclotide subfamilies Möbius
and bracelet. Three precursors differ significantly in these common processing sites; vibe 10, vibe 22
and vibe 25 lack the common asparagine or aspartic acid in their cyclotide domain at C termini, vital
for cyclisation. BLAST search of cyclisation enzyme, asparagine endopeptidase in the V. betonicifolia
transcriptome showed most similar to the predicted enzyme from V. uliginosa transcriptome (93%)
followed by butelase 1 (71%) and OaAEP1b (68%).
