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Title: | Enzyme-linked Immunosorbent Assay (ELISA) using recombinant protein antigens for detection of anti-chikungunya antibodies |
Authors: | Athapaththu, A.M.M.H. Khanna, N. Abeyewickreme, W. Gunasena, S. Hapugoda, M.D. |
Keywords: | Chikungunya virus Enzyme-Linked Immunosorbent Assay Enzyme-Linked Immunosorbent Assay-methods Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction |
Issue Date: | 2010 |
Publisher: | Faculty of Tropical Medicine, Mahidol University |
Citation: | Tropical Diseases Future Threats and New Paradigms, Proceedings of the Joint International Tropical Medicine Meeting & International Malaria Colloquium 2010 :103 |
Abstract: | OBJECTIVES: Chikungunya is a mosquito borne viral infection that has caused great medical and public health problems in South East Asia during last few years. Currently available laboratory diagnostic kits depend on Enzyme-Linked Immunosorbent Assay (ELISA) based on whole viral antigens caused biohazard risk, high production cost and cross reactivity with other organisms of the same genus/family. These problems can be avoided by using recombinant protein antigens in ELISAs. METHODOLOGY: Two novel recombinant protein antigens based on Envelope (E) domain, a critical antigenic region of the major structural protein of chikungunya virus were expressed separately in a bacterial expression system (Escherichia coli). Two proteins were purified under denatured conditions. They were evaluated as potential diagnostic intermediates for detection of and-chikungunya antibodies in Immunoglobulin M (IgM) and Immunoglobulin G (IgG) ELISAs separately using a panel of serum samples confirmed by the gold standard assay, Heamagglutination Inhibition (HAI) assayRESULTS: These 2 protein antigens: El and E2 showed more than 60% positivity in IgG ELISAs and IgM ELISAs. A field validation using a large number of serum samples should be done for further confirmation of these results. It can be concluded that these 2 novel recombinant protein antigens can be used as a diagnostic intermediate to detect anti-chikungunya antibodies. ACKNOWLEDGEMENTS: Financial assistance from the International Centre for Genetic Engineering and Biotechnology (1CGEB CRP/ SRI08-02) is gratefully acknowledged |
Description: | Oral Presentation Abstract of Joint International Tropical Medicine Meeting (JITMM 2010) & International Malaria Colloquium (IMC2010) 1-3 December 2010 Bangkok, Thailand |
URI: | http://repository.kln.ac.lk/handle/123456789/9360 |
Appears in Collections: | Conference Papers |
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