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dc.contributor.authorJayasooriya, D.H.S.W.
dc.contributor.authorGunawardene, Y.I.N.S.
dc.contributor.authorManamperi, A.
dc.contributor.authorHapugoda, M.D.
dc.contributor.authorPremaratna, R.
dc.identifier.citationTropical Medicine in the-Omics Era, Proceedings of the Joint International Tropical Medicine Meeting 2008; 101en_US
dc.descriptionOral Presentation of Joint International Tropical Medicine Meeting(JITMM 2008), 13-14 October 2008 Bangkok, Thailanden_US
dc.description.abstractDengue Virus (DENV) affecting millions of people mainly in the tropical and subtropical regions is a positive sense RNA virus which exists in four different serotypes (DENV1-4) and several subtypes. All four serotypes have been found in Sri Lanka. The objective of the study was to determine the DENV serotype distribution in patients and mosquitoes of Gampaha district. Serum was isolated from acute phase blood samples collected from dengue suspected patients during the febrile period. Mosquitoes were collected from different locations where dengue confirmed cases were reported. Pooled and crushed mosquito heads and serum samples were separately subjected to RNA extraction, RT-PCR, semi nested PCR with Dengue serotype specific primers and solution hybridization with Phosphorous 32 labeled probes respectively. Out of 93 patients, 34 were positive for DENV out of which 19 corresponded to DENV2, 5 to DENV3 and 10 were co-infected with DENV2 and DENV3. Of the positive infections corresponding to serotypes 2, 3, 2-3 co-infected; 7/19 (36.8%), 3/5(60%), 0/10 (0%) had developed dengue haernorrhagic fever respectively Among 14 mosquito pools, 5 were positive of which 3 were infected with DENV2 and 2 with DENV3. It was observed that although both DENV2 and DENV3 circulate among patients and in mosquitoes in Garnpaha District, DENV2 was predominant in both. Only Aedes albopictus 'presence in all localities was significant. A greater percentage of severe form of the disease among DENV3 infected patients was observed while a less severe form of the disease was observed in DENV2 and DENV3 co-infected patientsen_US
dc.publisherFaculty of Tropical Medicine, Mahidol Universityen_US
dc.subjectDengue Virusen_US
dc.subjectReverse Transcriptase Polymerase Chain Reaction-methodsen_US
dc.titleSerotype distribution of dengue in Gampaha District, Sri Lankaen_US
dc.typeConference Abstracten_US
Appears in Collections:Conference Papers

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