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Title: | Assessment of Bile Fluorescence Patterns in a Tropical Fish, Nile tilapia (Oreochromisniloticus) exposed to Naphthalene, Phenanthrene, Pyrene and Chrysene using Fixed Wavelength Fluorescence and Synchronous Fluorescence Spectrometry |
Authors: | Pathiratne, A. Hemachandra, C.K. Pathiratne, K.A.S. |
Keywords: | Tropical Fish Sri Lanka;Nile tilapia (Oreochromisniloticus);Phenanthrene; Pyrene (Chemical); Synchronous generators |
Issue Date: | 2010 |
Publisher: | Bulletin of Environmental Contamination and Toxicology |
Citation: | Bulletin of Environmental Contamination and Toxicology, May 2010, Volume 84, Issue 5, pp 554-558 |
Abstract: | Bile fluorescence patterns in Nile tilapia, a potential fish for biomonitoring tropical water pollution were assessed following exposure to selected polycyclic aromatic hydrocarbons (PAHs): naphthalene, phenanthrene, pyrene and chrysene. Non-normalized fixed wavelength fluorescence signals in the fish exposed to these PAHs reflected dose and/or time response relationships of their metabolism. Normalizing signals to biliverdin introduced deviations to these response patterns. The optimal wavelength pairs (excitation/emission) for synchronous fluorescence scanning measurements of bile metabolites of naphthalene, phenanthrene, pyrene and chrysene were identified as 284/326, 252/357, 340/382 and 273/382 respectively. This study supports the use of bile fluorescence in Nile tilapia by fixed wavelength fluorescence and synchronous fluorescence spectrometry with non-normalized data as a simple method for screening bioavailability of these PAHs. |
URI: | http://repository.kln.ac.lk/handle/123456789/4361 |
Appears in Collections: | Zoology |
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