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http://repository.kln.ac.lk/handle/123456789/24296
Title: | Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems |
Authors: | Rathnayake, I. V. N. Munagamage, T Pathirathne, A. Megharaj, M. |
Issue Date: | 2021 |
Publisher: | Water Science and Technology, 2021 |
Citation: | Rathnayake, I. V. N., Munagamage, T., Pathirathne, A., & Megharaj, M. (2021). Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems. Water Science and Technology, 84(7), 1579–1593. https://doi.org/10.2166/wst.2021.339 |
Abstract: | Bioavailable content of metals in aquatic systems has become critical in assessing the toxic effect of metals accumulating in the environment. Considering the need for rapid measurements, an optical microalgal-cyanobacterial array biosensor was developed using two strains of microalgae, Mesotaenium sp. and a strain of cyanobacteria Synechococcus sp. to detect Cd2+, Cr6+ and Zn2+ in aquatic systems. Microalgal and cyanobacterial cells were immobilized in a 96-well microplate using sol-gel method using silica. Optimum operational conditions for the biosensor array such as exposure time, storage stability, pH, and multiple metal effect were tested. A 10 min exposure time yielded optimum fluorescence values. Metal toxicity increased with decreasing pH, resulting in low relative fluorescence (%) and decreased with increasing pH, resulting in higher relative fluorescence (%). The optimum storage time for biosensor strains were 4 weeks for microalgal cultures and 8 weeks for cyanobacterial culture, at 4 °C storage temperature. The metal mixtures showed less effect on the inhibition of relative fluorescence (%) of microalgal/cyanobacterial cultures, displaying an antagonistic behavior among the metals tested. As a single unit, this photosynthetic array biosensor will be a valuable tool in detecting multi-metals in aquatic systems. |
URI: | http://repository.kln.ac.lk/handle/123456789/24296 |
Appears in Collections: | Articles |
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wst084071579.pdf | 948.78 kB | Adobe PDF | View/Open |
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