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DC Field | Value | Language |
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dc.contributor.author | Kaluarachchi, T.J. | |
dc.contributor.author | Wickremasinghe, R. | |
dc.contributor.author | Weerasekera, M. | |
dc.contributor.author | Yasawardene, S. | |
dc.contributor.author | McBain, A.J. | |
dc.contributor.author | Yapa, B. | |
dc.contributor.author | de Silva, H. | |
dc.contributor.author | Menike, C. | |
dc.contributor.author | Jayathilake, S. | |
dc.contributor.author | Munasinghe, A. | |
dc.contributor.author | Wickremasinghe, R. | |
dc.contributor.author | Ranasinghe, S. | |
dc.date.accessioned | 2021-05-05T06:59:09Z | |
dc.date.available | 2021-05-05T06:59:09Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Pathogens and Global Health. 2021;115(5): 307-314.[Epub 2021 Mar 9] | en_US |
dc.identifier.issn | 2047-7724 (Print) | |
dc.identifier.issn | 2047-7732 (Electronic) | |
dc.identifier.issn | 2047-7724 (Linking) | |
dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/22243 | |
dc.description | Indexed in MEDLINE | en_US |
dc.description.abstract | ABSTRACT: Cutaneous leishmaniasis (CL) is endemic in Sri Lanka. Giemsa-stained slit-skin-smears (SSS-Giemsa) and histology are routinely used in diagnosis with a sensitivity of 40-70%. PCR currently has limited accessibility. Therefore, we assessed the sensitivity and specificity of a previously described fluorescence in situ hybridization assay, on skin smears and biopsy samples to overcome the limitations encountered with routine diagnostic methods.Samples from a total of 123 suspected CL patients were collected and subjected to SSS-Giemsa, fluorescence in situ hybridization (FISH) on slit skin smears (SSS-FISH), formalin-fixed-paraffin-embedded-tissues stained with Hematoxylin & Eosin staining (FFPE-H&E) and FISH on formalin-fixed-paraffin-embedded-tissues (FFPE-FISH). Negative controls of 61 patient samples were collected from a CL non-endemic area and subjected to the same procedures. The gold standard PCR was used as a comparator. For FISH, two previously described cyanine 3 tagged Leihsmania genus-specific probes were used.Compared to PCR, SSS-Giemsa, SSS-FISH, FFPE-H&E, and FFPE-FISH had sensitivities of 76.5%, 79.1%, 50.4% and 80.9%, respectively. Routine diagnostic tests (SSS-Giemsa and FFPE-H&E) had a specificity of 100%. SSS-FISH and FFPE-FISH had specificities of 96.7% and 93.4%, respectively. FFPE-FISH had a statistically significant higher diagnostic performance than FFPE-H&E (p < 0.001). The relative performance of SSS-Giemsa, SSS-FISH and FFPE-FISH was similar (p > 0.05 for all comparisons).We conclude that FFPE-FISH is a more accurate diagnostic tool than FFPE-H&E. SSS-FISH did not have an additional advantage over SSS-Giemsa in diagnosis. However, SSS-FISH could be recommended as a minimally invasive method in studies assessing wound healing where immunological probes are used. KEYWORDS: Cutaneous leishmaniasis; Sri Lanka; fluorescence in situ hybridization. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Taylor & Francis Publishing | en_US |
dc.subject | cutaneous leishmaniasis | en_US |
dc.title | Diagnosing human cutaneous leishmaniasis using fluorescence in situ hybridization | en_US |
dc.type | Article | en_US |
Appears in Collections: | Journal/Magazine Articles |
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