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DC Field | Value | Language |
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dc.contributor.author | Niloofa, R. | |
dc.contributor.author | Karunanayake, L. | |
dc.contributor.author | de Silva, H.J. | |
dc.contributor.author | Premawansa, S. | |
dc.contributor.author | Rajapakse, S. | |
dc.contributor.author | Handunnetti, S. | |
dc.date.accessioned | 2021-02-16T07:28:49Z | |
dc.date.available | 2021-02-16T07:28:49Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | International Journal of Infectious Diseases. 2021; 105: 135-40. | en_US |
dc.identifier.issn | 1201-9712 (Print) | |
dc.identifier.issn | 1878-3511 (Electronic) | |
dc.identifier.issn | 1201-9712 (Linking) | |
dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/21989 | |
dc.description | Indexed in MEDLINE | en_US |
dc.description.abstract | BACKGROUND: Leptospirosis is most often clinically diagnosed and a laboratory test with high diagnostic accuracies is required. METHODOLOGY: IgM and IgG-ELISAs using Leptospira antigens were established and evaluated in relation to the Microscopic Agglutination Test (MAT). Antigen preparation consisted either saprophytic Leptospira biflexa to detect genus specific antibodies (genus-specific ELISA) or a pool of five most prevalent Leptospira interrogans serovars in Sri Lanka to detect serovar specific antibodies (serovar-specific ELISA). IgM and IgG immune responses in severe and mild leptospirosis patients (n = 100 in each group) were studied. RESULTS: ELISAs showed high repeatability and reproducibility. Serovar-specific IgM-ELISA showed sensitivity of 80.2% and specificity of 89%; genus-specific IgM-ELISA showed sensitivity of 83.3% and specificity of 91%. Serovar and genus-specific IgG-ELISA showed sensitivities of 73.3% and 81.7%, and specificities of 83.33%. Commercial IgM-ELISA showed sensitivity and specificity of 79.2% and 93% respectively. Commercial IgG-ELISA showed sensitivity, specificity of 50% and 96.7% respectively. IgM levels observed in mild and severe leptospirosis (ML & SL) patients were significantly higher than healthy control (HC) group, having absorbance mean of 0.770, 0.778 and 0.163 respectively. In contrast, SL patients had significantly higher mean anti-leptospiral IgG levels compared to both ML and HC groups (0.643, 0.358 and 0.116 respectively; ANOVA, P < 0.001). Presence of anti-leptospiral IgG above OD 0.643 optical density (OD) at 1:100 could predict high risk of severe disease. CONCLUSION: Serovar-specific In-House ELISAs could be used for the laboratory diagnosis of leptospirosis in endemic settings. Observed high levels of anti-leptospiral IgG suggest its value as a predictor for disease severity. KEYWORDS: IgM and IgG antibodies; In-House ELISA; Leptospirosis; Sero-diagnosis. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | ELISA | en_US |
dc.title | Development of an in-house ELISA as an alternative method for the serodiagnosis of leptospirosis | en_US |
dc.type | Article | en_US |
Appears in Collections: | Journal/Magazine Articles |
Files in This Item:
File | Description | Size | Format | |
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Development of an In-House ELISA.pdf | 2.18 MB | Adobe PDF | View/Open |
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