Please use this identifier to cite or link to this item: http://repository.kln.ac.lk/handle/123456789/21676
Title: Determination of total phenolic content (TPC) of Nymphaea nouchali and Nymphaea pubescens by Folin Ciocalteu method
Authors: Sundaram, T. M.
Napagoda, M. T.
Wijesekera, K. D.
Wijayaratne, Gaya Bandara
Keywords: Total Phenolic Content, Nymphaea nouchali, Nymphaea pubescens, Folin Ciocalteu
Issue Date: 2020
Publisher: Institute of Chemistry Ceylon Adamantane House, Rajagiriya, Sri Lanka.
Citation: Sundaram, T. M., Napagoda, M. T., Wijesekera, K. D. and Wijayaratne, Gaya Bandara(2020). Determination of total phenolic content (TPC) of Nymphaea nouchali and Nymphaea pubescens by Folin Ciocalteu method. In: International conference on Frontiers in Chemical Technology 2020. Institute of Chemistry Ceylon, p.58.
Abstract: Nymphaea nouchali (Nil Manel) and Nymphaea pubescens (Olu) are aquatic plants, and have a long history of use as food and folk medicine in many countries. Many phenolic compounds isolated from these two plant species have exhibited antioxidants properties and some simple phenolic compounds have shown to possess various pharmacological activities, including anti-inflammatory, antiproliferative, antioxidant and pro-apoptotic potential. The aim of the present study was to determine the content of total phenol in an extract from N. nouchali and N. pubescens flowers collected from Galle, Sri Lanka, by a UV/Vis spectrophotometric method. Aqueous methanol (80%) was used as the solvent to extract flowers including petals, stamens and pistil and all extracts were analyzed for TPC by Folin Ciocalteu method. Results were expressed in terms of gallic acid equivalence in (GAE)/g dry weight (DW) of flower part. N. pubescens petals contained 49.780 ± 0.626, stamens 37.581 ± 0.477 and pistil 31.889 ± 0.711, while N. nouchali petals showed 22.536 ± 0.383, stamens 38.702 ± 1.112 and pistil 30.119 ±1.116 GAEg-1. N. pubescens showed higher TPC in every plant part tested compared to N. nouchali and petal extract of N. pubescens showed highest TPC. Hence, N. pubescens flowers are a good source of antioxidants. Results emphasized that N. nouchali and N. pubescens extracts have high phenolic content and the importance of the use of natural products to treat pathological conditions.
URI: http://repository.kln.ac.lk/handle/123456789/21676
Appears in Collections:International conference on Frontiers in Chemical Technology 2020 (FCT 2020)



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