Standardization of Bakuladi Gandusha against the laboratory specimen of Staphylococcus aureus

Abstract

The Ayurveda and Traditional medical systems are used mainly by using herbal and mineral preparations for the treatment of diseases. Ayurveda recommends Gandüsha (mouthwash) as a specialized local therapy for good oral hygiene which maintains and promotes the oral hygiene by exerting the cleansing action to increasing the defense mechanism in the oral cavity. Medicinal plants included in herbal formulas are contained many types of anti- microbial compounds which is naturally occurring and having minimum side effects. In this study Staphylococcus aureus was used to check anti-microbial properties in standardization of Gandusha. According to the observations, Bakuladi Gandusha mostly used in Out Patient Department of Shalakya at Gampaha Wickramarachchi Ayurveda Teaching Hospital, Sri Lanka and has been clinically proved with successful results in patients of periodontal diseases. The main purpose of this study is to identify maximum effective concentration of this Gandusha, which have the anti-bacterial effect against staphylococcus aureus by Anti Bacterial Susceptibility Test (ABST). According to Gandusha paribhasha, Bakuladi decoction was diluted in to series of concentrations. Four samples of decoction on the bacterial strain were evaluated by using well diffusion method to evaluate the highest Inhibitory Zone Diameter (IZD)with in the concentration series for antimicrobial activity. The samples of Bakuladi Gandusha has got the positive antibacterial effect p<O.()5 at 95% level of significance on laboratory specimen of Staphylococcus aureus. But maximum mean inhibitory zone diameter was observed in sample D which has dilution is 12.67mm.