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DC Field | Value | Language |
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dc.contributor.author | Gunasekara, P. | - |
dc.contributor.author | Handunnetti, S.M. | - |
dc.contributor.author | Premawansa, S. | - |
dc.contributor.author | Kaluarachchi, P. | - |
dc.contributor.author | Karunatilake, C. | - |
dc.contributor.author | Ratnayake, I.P. | - |
dc.contributor.author | Dias, R. K. S. | - |
dc.contributor.author | Premakumara, G. A. S. | - |
dc.contributor.author | Dasanayake, W. M. D. K. | - |
dc.contributor.author | Seneviratne, S.L. | - |
dc.contributor.author | de Silva, R. | - |
dc.date.accessioned | 2020-01-27T09:53:15Z | - |
dc.date.available | 2020-01-27T09:53:15Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Gunasekara, P. Handunnetti, S.M. Premawansa, S. Kaluarachchi, P. Karunatilake, C. Ratnayake, I.P. Dias, R. K. S. Premakumara, G. A. S. Dasanayake, W. M. D. K. Seneviratne, S.L. and de Silva, R.(2019). Diagnosis of Vespa affinis venom allergy:use of immunochemical methods and a passive basophil activation test, Allergy, Asthma & Clinical Immunology. | en_US |
dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/20850 | - |
dc.description.abstract | Background: Allergy to Vespa affinis venom is common in the Asia Pacific region. Venom preparations for diagnosis are not commercially available for this species. Methods: The prominent allergens in V. affinis venom were identifiedusing immunochemical methods. Use of ImmunoCAP of Vespula vulgaris crude venom/its components and a passive basophil activation test (BAT) in the diagnosis of patients who had anaphylaxis to V. affinis venom (n = 30) were also accessed. The IgE double-positivity rates (positive to both hornet and honeybee) in ImmunoCAP and the passive BAT were determined. Results: High IgE reactivity was seen with the five allergens in V. affinis venom; 96% (29/30) for 34 and 24 kDa, 93% (28/30) for 45 kDa and 90% (27/30) reactivity for the 100 and 80 kDa respectively. IgE cross-reactivity was low with ImmunoCAP using V. vulgaris venom (43%; 13/30) and Ves v1 (3%; 1/30), but relatively high with Ves v5 (73%; 22/30). All patients (100%) were positive to V. affinis venom in passive BAT. In ImmunoCAP, a high double-positivity rate (76%; 23/30) was detected while no double-positivity was detected in passive BAT. Conclusions: High IgE reactivity for five allergens of V. affinis points to the potential of using these allergens in component resolved diagnosis (CRD). The passive BAT has shown its importance as a promising diagnostic tool with high accuracy. It would be particularly useful in cases with doubtful double-positive results of other diagnostic tests. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Allergy, Asthma & Clinical Immunology | en_US |
dc.subject | CD63 | en_US |
dc.subject | IgE cross-reactivity | en_US |
dc.subject | Insect venom allergy | en_US |
dc.subject | Passive basophil activation test | en_US |
dc.subject | Vespa affinis | en_US |
dc.title | Diagnosis of Vespa affinis venom allergy:use of immunochemical methods and a passive basophil activation test | en_US |
dc.type | Article | en_US |
Appears in Collections: | Zoology |
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