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Title: | Liquid chromatography-tandem mass spectrometry method for the simultaneous quantitative determination of the organophosphorus pesticides dimethoate, fenthion, diazinon and chlorpyrifos in human blood |
Authors: | Salm, P. Taylor, P. J. Roberts, D. de Silva, J. |
Issue Date: | 2009 |
Publisher: | Elsevier |
Citation: | Journal of Chromatography B. 2009; 877(5-6): pp.568-74 |
Abstract: | Simultaneous determination of the organophosphorus pesticides dimethoate, fenthion, diazinon and chlorpyrifos in human blood by HPLC–tandem mass spectrometry was developed and validated. The pesticides were extracted by a simple one-step protein precipitation procedure. Chromatography was performed on a Luna C18 (30 mm × 2.0 mm, 3 μm) column, using a step-gradient at a flow rate of 0.4 ml/min. The assay was linear from 0.5 to 100 ng/ml (r2 > 0.992, n = 24) for all pesticides. The inter- and intra-day accuracy and precision for the method was 96.6–106.1% and <10%, respectively. The lower limit of quantification was 0.5 ng/ml. In conclusion, the method described displays analytical performance characteristics that are suitable for the quantification of these pesticides in cases of acute poisoning. |
Description: | Indexed in mEDLINE |
URI: | http://repository.kln.ac.lk/handle/123456789/1909 |
ISSN: | 1570-0232 (Print) 1873-376X (Electronic) |
Appears in Collections: | Journal/Magazine Articles |
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