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DC Field | Value | Language |
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dc.contributor.author | Wijesooriya, L.I. | - |
dc.contributor.author | Kok, T. | - |
dc.contributor.author | Perera, J. | - |
dc.contributor.author | Tilakarathne, Y. | - |
dc.contributor.author | Sunil-Chandra, N.P. | - |
dc.date.accessioned | 2018-09-26T04:55:50Z | - |
dc.date.available | 2018-09-26T04:55:50Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Journal of medical microbiology.2018;67(9):1232-1242 | en_US |
dc.identifier.issn | 0022-2615 (Print) | - |
dc.identifier.issn | 1473-5644 (Electronic) | - |
dc.identifier.issn | 0022-2615 (Linking) | - |
dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/19048 | - |
dc.description | Indexed In MEDLINE | en_US |
dc.description.abstract | PURPOSE: Respiratory tract infections are a major cause of global morbidity and mortality. Pneumonia is the ninth leading cause of mortality in Sri Lanka. Atypical pathogens cause about one-fifth of community-acquired pneumonia, while Mycoplasma pneumoniae accounts for about 50 %. This study aimed to determine the seroprevalence of M. pneumoniae respiratory tract infections in Sri Lanka while attempting to understand the relationships between the serology and PCR. METHODOLOGY: Paired sera from 418 adult patients (pneumonia, n=97; bronchitis, n=183; pharyngitis, n=138) and 87 healthy controls were studied. IgM, IgG and IgA antibodies were tested by M. pneumoniae enzyme-linked immunosorbent assay (ELISA). Positive IgM and or IgG seroconversion was considered to be seropositive. M. pneumoniae DNA were tested by PCR in age and gender-matched seropositives and seronegatives. RESULTS: M. pneumoniae IgG was in 14.4 % (14/97), 6.0 % (11/183) and 1.5 % (2/138) of pneumonia, bronchitis and pharyngitis patients, respectively, whilst IgM was in 6.2 % (6/97), 1.1 % (2/183) and 0 % (0/138), respectively. Amongst the pneumonia seropositives, 64.7 % (11/17) showed IgG alone, 17.5 % (3/17) showed IgM alone and 17.5 % (3/17) showed IgM and IgG. Amongst the bronchitis seropositives, 84.6 % (11/13) had IgG alone and 15.4 % (2/13) had IgM alone. In the pharyngitis seropositives, only IgG was detected 100 % (2/2). M. pneumoniae DNA was in 52.2 % (12/23) of seropositives and 15.4 % (4/26) of seronegatives. In pneumonia or bronchitis patients, specific DNA was in 77.8 % (7/10) and 50 % (6/12) of patients, respectively. M. pneumoniae DNA was not found in pharyngitis patients. Of the seropositive PCR-negative pneumonia patients, 66.7 % (2/3) showed IgG alone and 33.3 % (1/3)showed IgM alone. In bronchitis patients, 83.3 % (5/6) showed IgG alone and 16.7 % (1/6) showed IgM alone. Of the seronegative PCR-positive patients, 16.7 % (2/12) had pneumonia and 18.2 % (2/11) had bronchitis. CONCLUSION: The serological evidence for M. pneumoniae infection in Sri Lanka comprised the following prevalences: 17.5 % (17/97), 7.1 % (13/183) and 1.4 % (2/138) in adults with pneumonia, bronchitis or pharyngitis, respectively. M. pneumoniae DNA was in 52.2 % (12/23) of seropositives and 15.4 % (4/26) of seronegatives. IgG was predominant in PCR positives and negatives. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Microbiology Society | en_US |
dc.subject | DNA detection | en_US |
dc.title | Mycoplasma pneumoniae DNA detection and specific antibody class response in patients from two tertiary care hospitals in tropical Sri Lanka | en_US |
dc.type | Article | en_US |
Appears in Collections: | Journal/Magazine Articles |
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