Please use this identifier to cite or link to this item: http://repository.kln.ac.lk/handle/123456789/16179
Title: Assessment of artificial blood feeding methods and efficacy of different blood meal sources in mass rearing of Aedes aegypti for innovative vector controlling strategies
Authors: Gunathilaka, P.A.D.H.N.
Ranathunga, R.M.T.B.
Udayanga, N.W.B.A.L.
Abeyewickreme, W.
Keywords: Aedes aegypti
blood feeding
techniques
blood meal
mass rearing
Issue Date: 2016
Publisher: Faculty of Graduate Studies, University of Kelaniya, Sri Lanka
Citation: Gunathilaka, P.A.D.H.N., Ranathunga, R.M.T.B., Udayanga, N.W.B.A.L. and Abeyewickreme, W. 2016. Assessment of artificial blood feeding methods and efficacy of different blood meal sources in mass rearing of Aedes aegypti for innovative vector controlling strategies. In proceedings of the 17th Conference on Postgraduate Research, International Postgraduate Research Conference 2016, Faculty of Graduate Studies, University of Kelaniya, Sri Lanka. p 247.
Abstract: Background Mass rearing of vector mosquitoes of dengue is a crucial requirement for implementation of innovative vector controlling strategies such as Sterile Insect Technique (SIT), Incompatible Insect Technique (IIT) and transgenic mosquitoes. Selection of the artificial membrane-feeding technique and blood meal source have been recognized as key considerations in mass rearing of vectors. Methodology Three artificial membrane feeding techniques (Glass plate method, Metal plate and Hemotek membrane feeding method) and three blood sources (human, cattle and chicken) were evaluated based on feeding rates, fecundity and hatching rates of Ae. aegypti, under laboratory conditions. One-way ANOVA, cluster analysis, analysis of variance (ANOSIM) and principal coordinates (PCO) analysis, were used to investigate the significance in the variations among blood feeding. Results Feeding rates of Ae. aegypti significantly differed among the membrane feeding techniques as suggested by one-way ANOVA (p <0.05). The metal plate method was identified as the most efficient and cost effective feeding technique. Blood feeding rate of Ae. aegypti was higher with human blood followed by cattle and chicken blood, respectively. However, no significant difference was observed among mosquitoes fed with cattle blood and human blood, in terms of fecundity, oviposition rate and fertility. Conclusions Metal plate feeding technique could be recommended as the most effective membrane feeding technique for mass rearing of Ae. aegypti, due to its high feeding rate and cost effectiveness. Since, obtaining human blood for insectary colony maintenance is problematic due certain ethical issues, cattle blood could be recommended for mass rearing Ae. aegypti.
URI: http://repository.kln.ac.lk/handle/123456789/16179
Appears in Collections:IPRC - 2016

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