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Title: | Protection against malaria in toque monkeys immunized with P. cynomolgi MSPI-19 in alum |
Authors: | Amaratunga, C. Nandasiri, K. Weerasinghe, S. Manamperi, A. Longacre, S. Holm, I. Handunnetti, S. |
Keywords: | malaria toque monkes |
Issue Date: | 2000 |
Publisher: | The royal society of tropical medicine and hygiene, British society for parasitology, The American society of tropical medicine and hygiene |
Citation: | Proceedings of the New challenges in tropical medicine and parasitology - Oxford. 2000 |
Abstract: | In a pre-clinical trail of a vaccine against P.vivax malaria, toque monkeys were immunized with matalloaffinity-purified baculovirus-expressed. His-tagged recombinant Plasmodium cynomolgy ceylonensis (Pcc) C-terminal 19 kDa protiens of the major merozoite surface protein 1 (MSP1p19) with alum, under conditions acceptable for clinical trials. The P. cynomolgi-toque monkey system is highly analogous to P.vivax in humans. Eight animals were immunized with alum+MSP1p19 and 3 monkeys received alum alone. After four doses, the anti-MSP1p19 antibody titres of immunized animals reached 2.8x104. all animals were challenged with Pcc asexual blood stage parasites. The immunized animals showed significant, partial protection (p=0.0024). Four of these animals were given a second heterologous challenge infection of p.cynomolgi Gombak, (PcG) four months after the first challenge infection. Sequence analysis of PcG DNA revealed a single amino acid challenge differing from that of Pcc. The substitution which occurs at the nt 207 position in the C-terminal 19-kDa sequence changes the amino acid glutamate into lysine. Statistically significant protection was observed in the immunized animals (p=0.04), despite having a lower titre of antibodies at the time of re-challenge. This documents the ability of recombinant MSP1p19 with alum to protect against Pcc and PcG infections. |
Description: | Abstract - Proceedings of the New challenges in tropical medicine and parasitology - Oxford 2000, 18-22 September, Oxford, UK. |
URI: | http://repository.kln.ac.lk/handle/123456789/15157 |
Appears in Collections: | Conference Papers |
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