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DC Field | Value | Language |
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dc.contributor.author | Thabrew, M.I. | en_US |
dc.contributor.author | Hughes, R.D. | en_US |
dc.contributor.author | Mcfarlane, I.G. | en_US |
dc.date.accessioned | 2014-10-29T09:15:08Z | |
dc.date.available | 2014-10-29T09:15:08Z | |
dc.date.issued | 1997 | en_US |
dc.identifier.citation | Journal of Pharmacy and Pharmacology. 1997; 49(11): 1132-1135 | en_US |
dc.identifier.issn | 0022-3573 (Print) | en_US |
dc.identifier.issn | 2042-7158 (Electronic) | en_US |
dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/1318 | |
dc.description | Indexed in MEDLINE | |
dc.description.abstract | Identification of the active components of plants with hepatoprotective properties requires screening of large numbers of samples during fractionation and purification. A screening assay has been developed based on protection of human liver-derived HepG2 cells against toxic damage. Various hepatotoxins were incubated with HepG2 cells in 96-well microtitre plates (30,000 cells well-1) for 1 h and viability was determined by metabolism of the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)-2-(4-sulphophenyl)-2H-tetrazolium (MTS). Bromobenzene (10 mM) and 2,6-dimethyl-N-acetyl-p-quinoneimine (2,6-diMeNAPQI, 200 mM) had greater toxic effects than tert-butyl hydroperoxide (1.8 mM) or galactosamine (10 mM), reducing mean viability to 44.6 +/- 1.2% (s.e.m.) and 56.1 +/- 2.1% of control, respectively. Protection against toxic damage by these agents was tested using a crude extract of a known hepatoprotective Sri Lankan plant, Osbeckia aspera, and two pure established hepatoprotective plant compounds, (+)-catechin and silymarin (1 mg mL-1). Viability was significantly improved by Osbeckia (by 37.7 +/- 2.4%, P < 0.05, and 36.5 +/- 2.1%, P < 0.05, for bromobenzene and 2,6-diMeNAPQI toxicity, respectively). Comparable values for (+)-catechin were 68.6 +/- 2.9% and 63.5 +/- 1.1%, and for silymarin 24.9 +/- 1.4% and 25.0 +/- 1.6%. This rapid and reproducible assay should prove useful for the isolation and identification of active hepatoprotective compounds in crude plant extracts. | |
dc.publisher | Wiley | en_US |
dc.subject | Plant Extracts | |
dc.subject | Plant Extracts-therapeutic use | |
dc.subject | Liver-drug effects | |
dc.subject | Cell Survival-drug effects | |
dc.subject | Drug Evaluation, Preclinical-methods | |
dc.subject | Bromobenzenes-toxicity | |
dc.subject | Catechin-therapeutic use | |
dc.subject | Silymarin-therapeutic use | |
dc.title | Screening of hepatoprotective plant components using a HepG2 cell cytotoxicity assay | en_US |
dc.type | Article | en_US |
dc.identifier.department | Biochemistry | en_US |
dc.creator.corporateauthor | Pharmaceutical Society of Great Britain | en_US |
dc.creator.corporateauthor | Royal Pharmaceutical Society of Great Britain | en_US |
dc.creator.corporateauthor | British Pharmaceutical Conference | en_US |
Appears in Collections: | Journal/Magazine Articles |
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