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Title: | Comparison of recombinant protein and cell lysate antigens for detection of anti-chikungunya (CHIK) IgM antibody |
Authors: | Athapaththu, A.M.M.H. Khanna, N. Inouve, S. Gunasena, S. Abeyewickreme, W. Hapugoda, M.D. |
Keywords: | chikungunya |
Issue Date: | 2011 |
Publisher: | Sri Lanka College of Microbiologists |
Citation: | The Bulletin of the Sri Lanka College of Microbiologists. 2011; 09(1): 16 |
Abstract: | INTRODUCTION: Chikungunya (CHIK) virus specific antigen which has high specificity and low cross reactivity with other related diseases is required for laboratory confirmation. OBJECTIVE: To compare two antigens for detection of anti-CHIK antibody. DESIGN, SETTING AND METHODS: In this study, two antigens {viral cell lysate and recombinant protein) were evaluated for detection of anti-1 CH IK antibody by using IgM ELISA. A novel recombinant | protein antigen was designed based on envelope domain, a critical antigenic region of the major structural protein, I This protein was expressed in Escherichia coli and resultant protein was affinity purified and ~10mg with >95% of purity per liter of culture was obtained. Cell lysate antigen was prepared using a crude culture fluid. Two antigens were evaluated separately using a panel of well characterized serum samples obtained from the Dept. of Virology (WHO Reference Centre for Viral Reference and Research), Institute of Tropical Medicine,] Nagasaki University. RESULTS: Atotal of 64 serum samples confirmed as positives andl 22 confirmed as negatives were used to evaluate the antigens. Specificity and sensitivity of the recombinant protein antigen was 48% and 90% respectively. Specificity and sensitivity of the viral lysate antigen was] 17% and 100% respectively. Conclusion Viral lysate antigens can cause biohazard risk, high production cost and cross reactivity with other organisms of the same genus/family. Recombinant protein antigen which shows high specificity and sensitivity used in this study is important to overcome problems associated with viral lysate antigen. Testing of a large number of samples is needed to reconfirm this finding. ACKNOWLEDGMENT: Financial assistance and technical co-operation by International Center for Genetic Engineering and Biotechnology (ICGEB CRP SRL 08/02), National Science Foundation (NSF/RG/2009/BT/01) and International Atomic Energy Authority (IAEA/SRL/5/042) is acknowledged. |
Description: | Oral Presentation (OP 6) The bulletin of the Sri Lanka College of Microbiologists, 04th-16th September 2011, Colombo |
URI: | http://repository.kln.ac.lk/handle/123456789/13184 |
ISSN: | 1391-930x |
Appears in Collections: | Conference Papers |
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