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DC Field | Value | Language |
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dc.contributor.author | Samarawickrema, N.A. | en_US |
dc.contributor.author | Brown, D.M. | en_US |
dc.contributor.author | Upcroft, J.A. | en_US |
dc.contributor.author | Thammapalerd, N. | en_US |
dc.contributor.author | Uproft, P. | en_US |
dc.date.accessioned | 2014-10-29T09:14:59Z | |
dc.date.available | 2014-10-29T09:14:59Z | |
dc.date.issued | 1997 | en_US |
dc.identifier.citation | The Journal of Antimicrobial Chemotherapy. 1997; 40: pp.833-840 | en_US |
dc.identifier.issn | 0305-7453 (Print) | en_US |
dc.identifier.issn | 1460-2091 (Electronic) | en_US |
dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/1304 | |
dc.description | Indexed in MEDLINE | |
dc.description.abstract | Metronidazole resistance has been induced in an axenic strain of Entamoeba histolytica (HTH-56:MUTM) following continuous exposure to steadily increasing drug concentrations. The drug-resistant line is routinely maintained in normally lethal levels of metronidazole (10 microM).Resistance to this concentration of drug was developed over 177 days. Decreased pyruvate:ferredoxin oxidoreductase (PFOR) activity in anaerobic organisms is one mechanism of metronidazole resistance but in entamoeba, PFOR activity was not decreased in metronidazole-resistant parasites as determined by immunofluorescent assays and immunoblotting studies. 2-Oxoacid oxidoreductase activity, which appeared to be due to a single enzyme, PFOR, was evident with pyruvate as well as the alternative substrates, alpha-ketobutyrate, alpha-ketoglutarate and oxaloacetate. A marked increase in superoxide dismutase (SOD) activity was detected in metronidazole-resistant E.histolytica. Increased SOD activity has not previously been documented as a mechanism of drug resistance although SOD has been associated with a range of stress situations in other organisms. | en_US |
dc.publisher | Oxford University Press | en_US |
dc.subject | Drug Resistance | en_US |
dc.subject | Entamoeba histolytica | en_US |
dc.subject | Entamoeba histolytica-drug effects | en_US |
dc.subject | Entamoeba histolytica-enzymology | en_US |
dc.subject | Ketone Oxidoreductases-metabolism | en_US |
dc.subject | Metronidazole | en_US |
dc.subject | Metronidazole-pharmacology | en_US |
dc.subject | Superoxide Dismutase-metabolism | en_US |
dc.subject | Comparative Study | en_US |
dc.title | Superoxide dismutase and pyruvate ferrodoxin oxidoreductase involvement in mechanisms of metronidazole resistance in Entamoeba histolytica | en_US |
dc.type | Article | en_US |
dc.identifier.department | Biochemistry | en_US |
dc.creator.corporateauthor | British Society for Antimicrobial Chemotherapy | en_US |
Appears in Collections: | Journal/Magazine Articles |
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