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DC Field | Value | Language |
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dc.contributor.author | Manamperi, N.H. | - |
dc.contributor.author | Oghumu, S. | - |
dc.contributor.author | Pathirana, K.P.N. | - |
dc.contributor.author | Munidasa, U.A.D.D. | - |
dc.contributor.author | Somaratne, K.K.V.N. | - |
dc.contributor.author | Rathnayake, R.M.D.I. | - |
dc.contributor.author | Thilakaratne, S.M. | - |
dc.contributor.author | de Silva, M.V.C. | - |
dc.contributor.author | Pathmeswaran, A. | - |
dc.contributor.author | Abeyewickreme, W. | - |
dc.contributor.author | Satoskar, A.R. | - |
dc.contributor.author | Karunaweera, N.D. | - |
dc.date.accessioned | 2016-05-02T07:18:56Z | - |
dc.date.available | 2016-05-02T07:18:56Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Proceedings of the 25th Anniversary International Scientific Conference. Faculty of Medicine, University of Kelaniya; 2016: 90 | en_US |
dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/12838 | - |
dc.description | Free paper session 2: Infections OP 9 - 25th Anniversary International Scientific Conference, 6-8 April 2016, Faculty of Medicine,University of Kelaniya, Sri Lanka | en_US |
dc.description.abstract | BACKGROUND: Cutaneous leishmaniasis (CL) in Sri Lanka is caused by the usually visceralizing Leishmania donovani. Host immune response plays a key role in the clinical presentation of leishmaniasis. Role of cytokines in pathogenesis of local lesions has not been studied. OBJECTIVE: To describe tissue cytokine expression with lesion progression with time in CL due to Leishmania donovani. METHODS: Skin biopsies from fifty eight patients with parasitologically or histopathologically confirmed CL and 30 healthy controls were analyzed for local tissue expression of Interleukin (IL)-12A, IL-4, IL-10, Interferon-gamma (IFNg) and Tumor Necrosis Factor-alpha (TNF-α). Cytokine mRNA was quantified by real-time RT- PCR using SYBR green. Relative copy numbers were calculated for each gene by 2-ΔΔCt method using β-actin as the reference gene and healthy controls as the calibrator. Spearman correlation was used to determine the correlation between cytokines and duration of active skin lesions. RESULTS: The study group consisted of 37 males (63.8%) and 21 females (36.2%) with a mean age of 35 (SD=12.05) years which ranged between 18-66 years. Mean duration of lesions was 6.8 (SD=9.10) months with a range of 1-48 months. The Spearman correlation coefficient for relative copy numbers and lesion duration was 0.220, 0.077, 0.073, 0.235 and 0.295 for IL-12A, IL-4, IL-10, IFNg and TNF-α respectively. There was a significant positive correlation between expression of TNF-α and lesion duration (p= 0.024). CONCLUSIONS: Localized expression of TNF-α increases with time in CL due to L. donovani in Sri Lanka, which indicates development of a pro-inflammatory immune response at the site of infection as the disease progresses. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Faculty of Medicine, University of Kelaniya, Sri Lanka | en_US |
dc.subject | cutaneous leishmaniasis | en_US |
dc.title | Pathogenesis of Leishmania donovani induced cutaneous leishmaniasis: role of Tumor Necrosis Factor α | en_US |
dc.type | Article | en_US |
Appears in Collections: | 25th Anniversary International Scientific Conference-2016 |
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