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Title: | A pilot study on comparison of rapid immunodiagnostics for confirmation of leptospirosis |
Authors: | Eugene, E.J. Wickramasinghe, S.A. Kalugalage, T.L. Rodrigo, C. Wickremesinghe, H. Dikmadugoda, N. Somaratne, P. de Silva, H.J. Rajapakse, S. Handunnetti, S.M. |
Keywords: | leptospirosis |
Issue Date: | 2012 |
Publisher: | Sri Lanka Medical Association |
Citation: | Sri Lanka Medical Association, 125th International Medical Congress. 2012;57 Suppliment1: 149 |
Abstract: | INTRODUCTION: In Sri Lanka, leptospirosis is mostly diagnosed on clinical grounds. Serological confirmation is not obtainable during the acute stage of the illness. There is a need for rapid immunodiagnostics for confirmation of leptospirosis. Two immunodiagnostic assays, ie: enzyme linkedimmnnosorbent assay (ELISA) and immunochromatographic technique Leptocheck-WB test (LCT) areused to detect leptospira specific IgM antibodies which are prevalent in early stages of acute infections. AIMS: To compare the efficacy of these two rapid immunodiagnostic assays with the microscopic agglutination assay (MAT) to determine their applicability. Methods: A set of sera (n=83) collected in 2010 for which MAT titres were available was used to perform IgMELISAandLCT. RESULTS: Positivity for LCT and IgM ELISA were 55.4% and 48.2% respectively, and both assays detected acute infection by day 3 of the illness. MAT> 400 was used as the reference standard. For LCT, the overall sensitivity, specificity, accuracy, PPV and NPV (86.5%, 75.0%, 79.6%, 69.6% and 89.4% respectively) were higher compared to the respective values for IgM ELISA (50.0%, 62.3%, 57.1%, 50.0%, 62.3%). The highest of these values were observed during the first week for LCT and during the second week for IgM ELISA. The highest agreement was observed between LCT and MAT>400 (p=0.568) and there was a good agreement between LCT and IgM ELISA (p=0.520). CONCLUSIONS: The high sensitivity and specificity, ease of use, and non-requirement of specialized skills and equipment makes LCT a good choice for screening, while IgM ELISA is an appropriate test for confirming acute leptopsirosis. |
Description: | Poster Presentation Abstract (PP 162), 125th Anniversary Scientific Medical Congress, Sri Lanka Medical Association, June 2012 Colombo, Sri Lanka |
URI: | http://repository.kln.ac.lk/handle/123456789/12426 |
ISSN: | 0009-0895 |
Appears in Collections: | Conference Papers |
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