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Title: | Cloning and characterization of a repetitive DNA sequence specific for Wuchereria bancrofti |
Authors: | Siridewa, K. Karunanayake, E.H. Chandrasekharan, N.V. Abeyewickreme, W. Franzen, L. Aslund, L. Pettersson, U. |
Keywords: | DNA, Helminth-chemistry Repetitive Sequences, Nucleic Acid Wuchereria bancrofti-genetics Wuchereria bancrofti |
Issue Date: | 1994 |
Publisher: | American Society of Tropical Medicine and Hygiene |
Citation: | The American Journal of Tropical Medicine and Hygiene. 1994; 51(4): pp.495-500 |
Abstract: | A genomic library constructed in a bacteriophage lambda replacement vector (EMBL3) with Wuchereria bancrofti DNA partially digested with Sau 3A I was screened with 32P-labeled W. bancrofti total DNA, and a strongly reactive recombinant, EMBL3Wb34, was isolated. This clone contained an approximately 16-kb insert that showed some cross-hybridization with Brugia malayi and B. pahangi DNA. However, a 969-bp subclone from EMBL3Wb34, designated pWb12, hybridized only with W. bancrofti DNA and was able to detect as little as 300 pg. Furthermore, pWb12 could detect DNA from a single infective larva or one microfilaria. It has a moderate copy number (450-700) and appears to be interspersed within the parasite genome. The nucleotide sequence contains 66% A+T and 34% G+C and shows no notable internal repeats. |
Description: | Indexed in MEDLINE |
URI: | http://repository.kln.ac.lk/handle/123456789/1228 |
ISSN: | 0002-9637 (Print) 1476-1645 (Electronic) |
Appears in Collections: | Journal/Magazine Articles |
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